首页> 生物试剂> 免疫检测> ELISA 检测与分析试剂盒


成功收藏产品
现货-m6A DNA甲基化定量检测试剂盒(比色法)-MethyFlash m6A DNA Methylation ELISA Kit (Colorimetric)
  • 市场价¥4,984.00好评率100%(0)条评论
    商城价 登录后可查看价格
  • 货号 P-9010-48
  • 品牌 Epigentek
  • CAS号
  • 规格/包装 48次
  • 售卖单位
  • 储存条件 -20
  • 现货状态 一个工作日

  • 购买数量

    - +

  • 立即购买
商家描述 产品评价(0)

m6A DNA甲基化定量检测试剂盒(比色法)                                                      货号:A-P-9010

MethyFlash m6A DNA Methylation ELISA Kit (Colorimetric)

   扫我收藏分享推荐,还有机会拿红包哦!

背景资料:N6-methyladenosine (m6A) is the most common and abundant modification on RNA molecules present in eukaryotes. DNA m6A is also identified in multicellular eukaryotes including Caenorhabditis elegans and Drosophila melanogaster, and furthermore identified in higher eukaryotes including plants, mouse and human cells. m6A plays crucial roles in regulating DNA replication, transposition, transcription, and cellular defense. In humans, the DNA m6A modification is most likely catalyzed by a methyltransferase complex METTL3 and removed by the α-ketoglutarate (α-KG)- and Fe2+-dependent dioxygenases such as ALKBH5 and TET-like enzymes. It was shown that METTL3 and α-KG /Fe2+-dependent dioxygenases play important roles in many biological processes, ranging from development and metabolism to fertility. The dynamic and reversible chemical m6A modification on DNA may also serve as a novel epigenetic marker of profound biological significance. Down-regulation of m6A modification was first characterized in human cancer cells and tissues, relative to their normal controls.  m6A is found to be the most regulated DNA modification in cancers. In addition to the regulation in cancer cells, relative to the primary cell/tissues which contain quite low amounts of DNA m6A (<0.001%), a hundreds-fold increase of m6A modification was found for in vitro cultured human cells (0.03%-0.22%). Therefore, identifying m6A DNA methylation levels and distribution on DNA could advance understanding of epigenetic regulation of biological process at the genomic level, and further provide useful information for improving diagnostics and therapeutics of disease

产品描述:This kit contains all reagents necessary for the quantification of m6A in DNA. In this assay, DNA is bound to strip wells using DNA high binding solution. m6A is detected using capture and detection antibodies. The detected signal is enhanced and then quantified colorimetrically by reading the absorbance in a microplate spectrophotometer. The amount of m6A is proportional to the OD intensity measured

产品特点:

Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 3 hours and 45 minutes

High sensitivity, of which the detection limit can be as low as 5 pg of m6A

3.Unique binding solution allows DNA >100 nts to be tightly bound to the wells, which enables quantification of m6A from both intact DNA or fragmented DNA such as ChIPed DNA

4.Optimized antibody and enhancer solutions allow high specificity to m6A, with no cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample DNA

5.Universal positive and negative controls are included, which are suitable for quantifying m6A from any species

6.Strip-well microplate format makes the assay flexible for manual or high throughput analysis

7.Simple, reliable, and consistent assay conditions

8.Flexible - Strip-well microplate format makes the assay available for manual or high throughput analysis

数据分析: 

操作流程 标准曲线 柱状图分析
m6A DNA甲基化定量检测试剂盒(比色法)操作步骤 将m6A标准品以不同浓度添加到孔中,使用m6A DNA甲基化定量检测试剂盒(比色法)来测 使用m6A DNA甲基化定量检测试剂盒(比色法)精确定量来自人类不同样本DNA的m6A 水平。结果与MS-LC一致。甚至更好。

 参考文献:

1.Lewinska A et. al. (February 2017). Downregulation of Methyltransferase Dnmt2 Results in Condition-dependent Telomere Shortening and Senescence or Apoptosis in Mouse Fibroblasts. J Cell Physiol.

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分。

定购信息:    
产品名称 规格 操作手册 货号 询价
m6A DNA甲基化定量检测试剂盒(比色法) 96次 英文PDF-中文PDF A-P-9010-96 点击这里给我发消息
m6A DNA甲基化定量检测试剂盒(比色法) 48次 英文PDF-中文PDF A-P-9010-48  
尿液样本 m6A 定量检测试剂盒(比色法) 96次 英文PDF-中文PDF A-P-9015-96 点击这里给我发消息
尿液样本 m6A 定量检测试剂盒(比色法) 48次 英文PDF- A-P-9015-48  
总体RNA甲基化极易定量检测试剂盒(荧光法) 96次 英文PDF-中文PDF A-P-9009-96 点击这里给我发消息
总体RNA甲基化极易定量检测试剂盒(荧光法) 48次 英文PDF-中文PDF A-P-9009-48  
总组蛋白H3K27乙酰化定量检测试剂盒(比色法) 48次 PDF A-P-4059-48  
总组蛋白H3K27乙酰化定量检测试剂盒(比色法) 96次 PDF A-P-4059-96  
EpiNext 高灵敏重亚硫酸盐测序试剂盒(Illumina) 12次 PDF A-P-1056A-12
EpiNext 高灵敏重亚硫酸盐测序试剂盒(Illumina) 24次 PDF A-P-1056A-24 点击这里给我发消息
96孔DNA甲基化极速修饰试剂盒(磁珠法)  96次 英文PDF-中文PDF A-P-1050-096  
96孔DNA甲基化极速修饰试剂盒(磁珠法)  192次 英文PDF-中文PDF A-P-1050-192  
抗人C5a/C5a desArg单克隆抗体(克隆号2942) 100ug
PDF
C-HM2078  
抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 100ug
PDF
C-HM2022F  
抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 50ug
PDF
C-HM2008  
抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 50ug
PDF
C-HM2006  
抗小鼠TREM-2单克隆抗体(克隆号6E9) 100ug PDF  C-HM1129F 点击这里给我发消息
m6A RNA甲基化定量检测试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-9005-96  
RNA甲基化修饰试剂盒 50 次 英文PDF--中文PDF A-P-9003-050  
EZ RNA甲基化修饰试剂盒 50 次 英文PDF--中文PDF A-R5001  

表观遗传学产品全面解决方案列下:   

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1036-96  
DNA羟甲基化定量试剂盒(荧光法) 48 次 英文PDF--中文PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 英文PDF--中文PDF A-P-1037-96  
DNA甲基化定量试剂盒(荧光法) 48 次 英文PDF--中文PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 英文PDF--中文PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1034-96  
总体DNA甲基化极易定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1030-48 点击这里给我发消息
总体DNA甲基化极易定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1030-96  
总体DNA羟甲基化极易定量试剂盒(比色法) 48 次 英文PDF--中文PDF A-P-1032-48 点击这里给我发消息
总体DNA羟甲基化极易定量试剂盒(比色法) 96 次 英文PDF--中文PDF A-P-1032-96  
总DNA甲基化定量试剂盒 96 次 英文PDF--中文PDF A-P-1014B-96  
咨询平台 我要吐槽 返回顶部