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β-Actinis one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actinare useful as loading controls for Western Blotting. However it should be noted that levels of β-Actinmay not be stable in certain cells. For example, expression of β-Actinin adipose tissue is very low and therefore β-Actinshould not be used as loading control for these tissues.
Product Name:β-Actinmouse monoclonal antibody
Clone Number: 6B8
Immunogen: Full-length β-Actin protein of human.
Tested applications: WB IHC IF
Recommended dilutions: WB: 1:5000-2000 IHC: 1:200-100 IF: 1:200-100
Optimal dilutions/concentrations should be determined by the end user.
Molecular Weight: 43KD
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: β-Actin antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Reactivity:Human, Monkey, Mouse, Rat, hamster, Dog, Spodoptera frugiperda, chicken, Zebrafish
Western blot analysis of Hela cell lysate(lane1) , Rat brain tissue lysate(lane2) and Mouse brain tissue lysate(lane3) with β-Actin mouse mAb(6B8) diluted at 1:5000.
Immunohistochemistry Staining of Human ovary tissue with β-Actin mouse mAb(6B8) diluted at 1:200.