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MILLIPORE PVDF膜0.22um，ISEQ00010
  • 市场价￥3,250.00好评率100%(0)条评论
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  • 货号 ISEQ00010
  • 品牌 Merck millipore/默克密理博
  • CAS号
  • 规格/包装 26.5cm×3.75m/卷
  • 售卖单位 卷
  • 储存条件
  • 现货状态 三个工作日
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重要规格表

主要应用
WB
描述
产品目录编号 ISEQ00010
商名
  • Immobilon
描述 Immobilon-PSQ 卷膜，PVDF，0.2 µm，26.5 cm x 3.75 m
背景信息 Overview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.
Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa
Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.
Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.
Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins
Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications
Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.
Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times
Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
参考
产品信息
检测方法 Chemiluminescence Chromogenic Radioactive
相容的染色剂
  • 考马斯亮蓝
  • 酰胺黑
  • 印度墨水
  • 丽春红
  • 胶体金
  • CPTS
  • 甲苯胺蓝
  • 透射显影
  • Sypro® 宝石红
过滤器代码 ISEQ
过滤器颜色 白色
应用
应用 低分子量 Western blotting、氨基酸分析和
N 端蛋白质测序
主要应用
  • Western Blotting
生物信息
主结合机制 静电，疏水
BSA吸收性 340 µg/cm²
山羊IgG吸收性 448 µg/cm²
胰岛素吸收性 262 µg/cm²
可湿润性 疏水
理化信息
孔径 0.2 µm
尺寸
过滤器表面 光面
过滤器尺寸 26.5 cm x 3.75 m
材料信息
化学
  • 疏水性聚偏氟乙烯（PVDF）

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