Summary  Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5).

UniPort summary Function  

Alternate names  UBC; HMG20; Ubiquitin;

Gene_symbol  

UniProt Number  P0CG48

GeneBank ID  

Gene ID  7316

Western blot analysis of extracts of HepG2 and HeLa cell line, using UBC antibody.

Immunohistochemistry of paraffin-embedded human liver using UBC antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of FFPE section of human breast carcinoma using UBC antibody.