Product Name          JC Polyomavirus IgG (JC-IgG), ELISA Kit     Also Known As          Qualitative Human JC Polyomavirus IgG (JC-IgG) ELISA Kit     Product Gene Name          JC-IgG elisa kit    [Similar Products]     Research Use Only          For Research Use Only. Not for use in diagnostic procedures.    TOP      Manual Insert                    Species Reactivity          Human             Samples          Serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.     Assay Type          Sandwich     Intended Uses          For the qualitative determination of activated Human JC Polyomavirus IgG (JC-IgG) in serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.     Intra-assay Precision          Intra-assay CV (%) are less than 15%.     Inter-assay Precision          Inter-assay CV (%) are less than 15%.         Preparation and Storage          Store all reagents at 2-8 degree C     Other Notes          Small volumes of JC-IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.     Searchable Terms for JC-IgG purchase          MBS109012 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the JC Polyomavirus IgG (JC-IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range in biological research samples containing JC-IgG. The ELISA analytical biochemical technique of the MBS109012 kit is based on JC-IgG antibody-JC-IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect JC-IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, JC-IgG. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenate, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).    TOP         Product Description specifically for JC-IgG elisa kit          Principle of the Assay: The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human JC-IgG in samples. Add sample to wells pre-coated with one Human JC Polyomavirus IgG antigen, at same time add HRP-conjugated Human JC Polyomavirus IgG antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human JC-IgG in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.