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Isoschizomers

Restriction endonucleases that recognize the same sequence are isoschizomers. The first example discovered is called a prototype and all subsequent enzymes that recognize the same sequence are isoschizomers of the prototype. The list below contains isoschizomers for commercially available restriction endonucleases. It also specifies which isoschizomer is available from New England Biolabs.

All recognition sequences are written 5´ to 3´ using the single letter code nomenclature with the point of cleavage indicated by a "/".

Numbers in parentheses indicate point of cleavage for non-palindromic enzymes. For example, GGTCTC(1/5) indicates cleavage at:
5´ ...GGTCTCN/...3´
3´ ...CCAGAGNNNNN/...5´

Isoschizomers with alternative cleavage sites are indicated with a " ^ ". Enzymes that are not currently commercially available are indicated with a " x ".

Neoschizomers are a subset of isoschizomers that recognize the same sequence, but cleave at different positions from the prototype. Thus, AatII (recognition sequence: GACGT↓C) and ZraI (recognition sequence: GAC↓GTC) are neoschizomers of one another, while HpaII (recognition sequence: C↓CGG) and MspI (recognition sequence: C↓CGG) are isoschizomers. Analogous designations are not appropriate for MTases, where the differences between enzymes are not so easily defined and usually have not been well characterized.


(1) A HF version of this enzyme is available.
(2) A RE-Mix version of this enzyme is available.

Enzyme Sequence NEB Enzyme Catalog Number Cut Site Other Isoschizomers
AanI TTA/TAA PsiI R0657 TTA/TAA PsiI
AarI CACCTGC(4/8)
AasI GACNNNN/NNGTC DrdI R0530 GACNNNN/NNGTC DrdI, DseDI
AatI AGG/CCT StuI R0187 AGG/CCT Eco147I, PceI , SseBI, StuI
AatII GACGT/C AatII R0117 GACGT/C ZraI^
ZraI^ R0659 GAC/GTC
AauI T/GTACA BsrGI R0575 T/GTACA Bsp1407I, BsrGI, BstAUI, SspBI
AbsI CC/TCGAGG
Acc113I AGT/ACT ScaI (1) (2) R0122 AGT/ACT AssI , BmcAI^, ScaI, ZrmI
Acc16I TGC/GCA FspI R0135 TGC/GCA AviII , FspI, MstI, NsbI
Acc36I ACCTGC(4/8) BfuAI R0701 ACCTGC(4/8) BfuAI, BspMI, BveI
BspMI R0502 ACCTGC(4/8)
Acc65I G/GTACC Acc65I R0599 G/GTACC Asp718I , KpnI^
KpnI^ (1) (2) R0142 GGTAC/C
AccB1I G/GYRCC BanI R0118 G/GYRCC BanI, BshNI, BspT107I, HgiCI
AccB7I CCANNNN/NTGG PflMI R0509 CCANNNN/NTGG BasI , PflBI , PflMI, Van91I
AccBSI CCGCTC(-3/-3) BsrBI R0102 CCGCTC(-3/-3) BsrBI, MbiI
AccI GT/MKAC AccI R0161 GT/MKAC FblI, XmiI
AccII CG/CG BstUI R0518 CG/CG Bsh1236I, BspFNI^, BstFNI, BstUI, FnuDII^, MvnI, ThaI
AccIII T/CCGGA BspEI R0540 T/CCGGA Aor13HI , BlfI , BseAI, BsiMI, Bsp13I, BspEI, BspMII, Kpn2I , MroI
AceIII x CAGCTC(7/11)
AciI CCGC(-3/-1) AciI R0551 CCGC(-3/-1) BspACI, SsiI
AclI AA/CGTT AclI R0598 AA/CGTT Psp1406I
AclWI GGATC(4/5) AlwI R0513 GGATC(4/5) AlwI, BinI, BspPI, Nt.AlwI^
Nt.AlwI^ R0627 GGATC(4/-5)
AcoI Y/GGCCR EaeI R0508 Y/GGCCR CfrI, EaeI
AcsI R/AATTY ApoI R0566 R/AATTY ApoI, XapI
AcuI CTGAAG(16/14) AcuI R0641 CTGAAG(16/14) Eco57I
AcvI CAC/GTG PmlI R0532 CAC/GTG BbrPI , Eco72I, PmaCI , PmlI, PspCI
AcyI GR/CGYC BsaHI R0556 GR/CGYC BsaHI, BssNI, BstACI, Hin1I, Hsp92I
AdeI CACNNN/GTG DraIII (1) R0510 CACNNN/GTG DraIII
AfaI GT/AC CviQI^ R0639 G/TAC Csp6I^, CviQI^, RsaI
RsaI R0167 GT/AC
AfeI AGC/GCT AfeI R0652 AGC/GCT Aor51HI , Eco47III, FunI
AfiI CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG Bsc4I, BseLI, BsiYI, BslI
AflI G/GWCC AvaII R0153 G/GWCC AvaII, Bme18I, Eco47I, PspO3I^, SinI, Vpak11AI^
AflII C/TTAAG AflII R0520 C/TTAAG BfrI , BspTI, Bst98I, BstAFI, MspCI, Vha464I
AflIII A/CRYGT AflIII R0541 A/CRYGT
AgeI A/CCGGT AgeI (1) (2) R0552 A/CCGGT AsiAI , BshTI, CspAI, PinAI
AgsI TTS/AA
AhaIII x TTT/AAA DraI^ R0129 TTT/AAA DraI^
AhdI GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AspEI , BmeRI, DriI, Eam1105I, EclHKI, NruGI
AhlI A/CTAGT SpeI (1) (2) R0133 A/CTAGT BcuI , SpeI
AjiI CACGTC(-3/-3) BmgBI R0628 CACGTC(-3/-3) BmgBI, BtrI^
AjnI /CCWGG BstNI^ R0168 CC/WGG BciT130I^, BptI^, BseBI^, BsiLI^, Bst2UI^, BstNI^, BstOI^, EcoRII^, MvaI^, Psp6I ^, PspGI^
PspGI^ R0611 /CCWGG
AjuI (7/12)GAANNNNNNNTTGG(11/6)
AleI CACNN/NNGTG AleI R0634 CACNN/NNGTG OliI
AlfI (10/12)GCANNNNNNTGC(12/10)
AloI (7/12)GAACNNNNNNTCC(12/7)
AluBI AG/CT
AluI AG/CT AluI R0137 AG/CT
Alw21I GWGCW/C BsiHKAI R0570 GWGCW/C AspHI , Bbv12I , BsiHKAI, HgiAI
Alw26I GTCTC(1/5) BcoDI R0542 GTCTC(1/5) BcoDI, BsmAI, BsoMAI, BstMAI, Nt.BsmAI^
BsmAI R0529 GTCTC(1/5)
Nt.BsmAI^ R0121 GTCTC(1/-5)
Alw44I G/TGCAC ApaLI R0507 G/TGCAC ApaLI, VneI
AlwFI x GAAAYNNNNNRTG
AlwI GGATC(4/5) AlwI R0513 GGATC(4/5) AclWI , BinI, BspPI, Nt.AlwI^
Nt.AlwI^ R0627 GGATC(4/-5)
AlwNI CAGNNN/CTG AlwNI R0514 CAGNNN/CTG CaiI, PstNI
Ama87I C/YCGRG AvaI R0152 C/YCGRG AvaI, BcoI , BmeT110I, BsiHKCI, BsoBI, Eco88I, NspIII
BsoBI R0586 C/YCGRG
AocI CC/TNAGG Bsu36I R0524 CC/TNAGG AxyI , Bse21I, Bsu36I, CvnI, Eco81I, SauI
Aor13HI T/CCGGA BspEI R0540 T/CCGGA AccIII, BlfI , BseAI, BsiMI, Bsp13I, BspEI, BspMII, Kpn2I , MroI
Aor51HI AGC/GCT AfeI R0652 AGC/GCT AfeI, Eco47III, FunI
AoxIx /GGCC HaeIII^ R0108 GG/CC BshFI ^, BsnI^, BspANI^, BsuRI^, HaeIII^, PalI ^, PhoI^
PhoI^ R0705 GG/CC
ApaBIx GCANNNNN/TGC BstAPI^ R0654 GCANNNN/NTGC BstAPI^
ApaI GGGCC/C ApaI R0114 GGGCC/C Bsp120I^, PspOMI^
PspOMI^ R0653 G/GGCCC
ApaLI G/TGCAC ApaLI R0507 G/TGCAC Alw44I , VneI
ApeKI G/CWGC ApeKI R0643 G/CWGC TseI
TseI R0591 G/CWGC
ApoI R/AATTY ApoI R0566 R/AATTY AcsI , XapI
ApyPIx ATCGAC(20/18)
AquIIx GCCGNAC(20/18)
AquIVx GRGGAAG(19/17)
ArsI (8/13)GACNNNNNNTTYG(11/6)
AscI GG/CGCGCC AscI (2) R0558 GG/CGCGCC PalAI , SgsI
AseI AT/TAAT AseI R0526 AT/TAAT AsnI , PshBI , VspI
AsiAI A/CCGGT AgeI (1) (2) R0552 A/CCGGT AgeI, BshTI, CspAI, PinAI
AsiGI A/CCGGT AgeI (1) (2) R0552 A/CCGGT AgeI, AsiAI , BshTI, CspAI, PinAI
AsiSI GCGAT/CGC AsiSI R0630 GCGAT/CGC RgaI, SfaAI, SgfI
AsnI AT/TAAT AseI R0526 AT/TAAT AseI, PshBI , VspI
Asp700I GAANN/NNTTC XmnI R0194 GAANN/NNTTC MroXI, PdmI , XmnI
Asp718I G/GTACC Acc65I R0599 G/GTACC Acc65I, KpnI^
KpnI^ (1) (2) R0142 GGTAC/C
AspA2I C/CTAGG AvrII R0174 C/CTAGG AvrII, BlnI , BspA2I, XmaJI
AspBHIx YSCNS(8/12)
AspCNI x GCCGC
AspEI GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AhdI, BmeRI, DriI, Eam1105I, EclHKI, NruGI
AspHI GWGCW/C BsiHKAI R0570 GWGCW/C Alw21I , Bbv12I , BsiHKAI, HgiAI
AspI GACN/NNGTC PflFI R0595 GACN/NNGTC PflFI, PsyI, TelI, Tth111I
Tth111I R0185 GACN/NNGTC
AspLEI GCG/C HhaI R0139 GCG/C BstHHI, CfoI, GlaI^, HhaI, R9529^, HinP1I^, HspAI ^
HinP1I^ R0124 G/CGC
AspS9I G/GNCC Sau96I R0165 G/GNCC AsuI , BmgT120I^, BsiZI, BspLI^, Cfr13I, FmuI^, PspPI , Sau96I, UnbI^
AssI x AGT/ACT ScaI (1) (2) R0122 AGT/ACT Acc113I , BmcAI^, ScaI, ZrmI
Asu14238IV CGTRAC
AsuC2I CC/SGG NciI R0196 CC/SGG BcnI , BpuMI, CauII, EcoHI^, NciI
AsuHPI GGTGA(8/7) HphI R0158 GGTGA(8/7) HphI
AsuI x G/GNCC Sau96I R0165 G/GNCC AspS9I , BmgT120I^, BsiZI, BspLI^, Cfr13I, FmuI^, PspPI , Sau96I, UnbI^
AsuII TT/CGAA BstBI R0519 TT/CGAA Bpu14I, BsiCI, Bsp119I, BspT104I, BstBI, Csp45I, NspV , SfuI
AsuNHI G/CTAGC BmtI^ (1) R0658 GCTAG/C BmtI^, BspOI^, NheI
NheI (1) (2) R0131 G/CTAGC
AvaI C/YCGRG AvaI R0152 C/YCGRG Ama87I , BcoI , BmeT110I, BsiHKCI, BsoBI, Eco88I, NspIII
BsoBI R0586 C/YCGRG
AvaII G/GWCC AvaII R0153 G/GWCC AflI , Bme18I, Eco47I, PspO3I^, SinI, Vpak11AI^
AvaIII x ATGCAT NsiI^ R0127 ATGCA/T BfrBl^, EcoT22I^, Mph1103I^, NsiI^, Zsp2I^
AviII TGC/GCA FspI R0135 TGC/GCA Acc16I , FspI, MstI, NsbI
AvrII C/CTAGG AvrII R0174 C/CTAGG AspA2I , BlnI , BspA2I, XmaJI
Awo1030IV GCCRAG
AxyI CC/TNAGG Bsu36I R0524 CC/TNAGG AocI , Bse21I, Bsu36I, CvnI, Eco81I, SauI
BaeGI GKGCM/C BaeGI R0708 GKGCM/C BseSI, BstSLI
BaeI (10/15)ACNNNNGTAYC(12/7) BaeI R0613 (10/15)ACNNNNGTAYC(12/7)
BalI TGG/CCA MscI R0534 TGG/CCA MlsI, MluNI, MscI, Msp20I
BamHI G/GATCC BamHI (1) R0136 G/GATCC
BanI G/GYRCC BanI R0118 G/GYRCC AccB1I , BshNI, BspT107I, HgiCI
BanII GRGCY/C BanII R0119 GRGCY/C Eco24I, EcoT38I, FriOI, HgiJII
BanIII AT/CGAT BspDI R0557 AT/CGAT Bsa29I, BseCI, BshVI, BsiXI, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI
ClaI R0197 AT/CGAT
BanLI RTCAGG
BarI (7/12)GAAGNNNNNNTAC(12/7)
BasI x CCANNNN/NTGG PflMI R0509 CCANNNN/NTGG AccB7I , PflBI , PflMI, Van91I
BauI CACGAG(-5/-1) BssSI R0587 CACGAG(-5/-1) BsiI, BssSI, Bst2BI
BbeI GGCGC/C KasI^ R0544 G/GCGCC DinI^, EgeI^, KasI^, Mly113I^, NarI^, PluTI, SfoI^, SspDI^
NarI^ R0191 GG/CGCC
PluTI R0713 GGCGC/C
SfoI^ R0606 GGC/GCC
BbrPI CAC/GTG PmlI R0532 CAC/GTG AcvI , Eco72I, PmaCI , PmlI, PspCI
BbsI GAAGAC(2/6) BbsI R0539 GAAGAC(2/6) BbvII , BpiI, BpuAI, BstV2I
BbuI GCATG/C SphI (1) R0182 GCATG/C PaeI , SpaHI, SphI
Bbv12I GWGCW/C BsiHKAI R0570 GWGCW/C Alw21I , AspHI , BsiHKAI, HgiAI
BbvCI CCTCAGC(-5/-2) BbvCI R0601 CCTCAGC(-5/-2) Nb.BbvCI^, Nt.BbvCI^
Nb.BbvCI^ R0631 CCTCAGC
Nt.BbvCI^ R0632 CCTCAGC(-5/-7)
BbvI GCAGC(8/12) BbvI R0173 GCAGC(8/12) BseXI, Bst71I, BstV1I, R9896
BbvII x GAAGAC(2/6) BbsI R0539 GAAGAC(2/6) BbsI, BpiI, BpuAI, BstV2I
BccI CCATC(4/5) BccI R0704 CCATC(4/5)
Bce83I x CTTGAG(16/14) BpuEI R0633 CTTGAG(16/14) BpuEI
BceAI ACGGC(12/14) BceAI R0623 ACGGC(12/14)
BcefI x ACGGC(12/13)
BcgI (10/12)CGANNNNNNTGC(12/10) BcgI R0545 (10/12)CGANNNNNNTGC(12/10)
BciT130I CC/WGG BstNI R0168 CC/WGG AjnI ^, BptI, BseBI, BsiLI, Bst2UI, BstNI, BstOI, EcoRII^, MvaI, Psp6I ^, PspGI^
PspGI^ R0611 /CCWGG
BciVI GTATCC(6/5) BciVI R0596 GTATCC(6/5) BfuI , BsuI
BclI T/GATCA BclI R0160 T/GATCA BsiQI, FbaI, Ksp22I
BcnI CC/SGG NciI R0196 CC/SGG AsuC2I , BpuMI, CauII, EcoHI^, NciI
BcoDI GTCTC(1/5) BcoDI R0542 GTCTC(1/5) Alw26I , BsmAI, BsoMAI, BstMAI, Nt.BsmAI^
BsmAI R0529 GTCTC(1/5)
Nt.BsmAI^ R0121 GTCTC(1/-5)
BcoI C/YCGRG AvaI R0152 C/YCGRG Ama87I , AvaI, BmeT110I, BsiHKCI, BsoBI, Eco88I, NspIII
BsoBI R0586 C/YCGRG
BcuI A/CTAGT SpeI (1) (2) R0133 A/CTAGT AhlI , SpeI
BdaIx (10/12)TGANNNNNNTCA(12/10)
BetI x W/CCGGW BsaWI R0567 W/CCGGW BsaWI
BfaI C/TAG BfaI R0568 C/TAG FspBI, MaeI, XspI
BfaSII GANGGAG
BfiI x ACTGGG(5/4) BmrI R0600 ACTGGG(5/4) BmrI, BmuI
BfmI C/TRYAG SfcI R0561 C/TRYAG BpcI, BstSFI, SfcI
BfoI RGCGC/Y HaeII R0107 RGCGC/Y Bsp143II, BstH2I, HaeII
BfrBlx ATGCA/T NsiI R0127 ATGCA/T AvaIII ^, EcoT22I, Mph1103I, NsiI, Zsp2I
BfrI C/TTAAG AflII R0520 C/TTAAG AflII, BspTI, Bst98I, BstAFI, MspCI, Vha464I
BfuAI ACCTGC(4/8) BfuAI R0701 ACCTGC(4/8) Acc36I , BspMI, BveI
BspMI R0502 ACCTGC(4/8)
BfuCI /GATC BfuCI R0636 /GATC BscFI, Bsp143I, BssMI, BstENII, BstKTI^, BstMBI, DpnI^, Kzo9I, MalI^, MboI, NdeII^, Sau3AI
DpnI^ R0176 GA/TC
MboI R0147 /GATC
Sau3AI R0169 /GATC
BfuI GTATCC(6/5) BciVI R0596 GTATCC(6/5) BciVI, BsuI
BglI GCCNNNN/NGGC BglI R0143 GCCNNNN/NGGC
BglII A/GATCT BglII R0144 A/GATCT
BinIx GGATC(4/5) AlwI R0513 GGATC(4/5) AclWI , AlwI, BspPI, Nt.AlwI^
Nt.AlwI^ R0627 GGATC(4/-5)
BisI GC/NGC Fnu4HI R0178 GC/NGC BlsI ^, BthCI^, Fnu4HI, Fsp4HI, GluI, ItaI , SatI
BlfI T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI , BseAI, BsiMI, Bsp13I, BspEI, BspMII, Kpn2I , MroI
BlnI C/CTAGG AvrII R0174 C/CTAGG AspA2I , AvrII, BspA2I, XmaJI
BlpI GC/TNAGC BlpI R0585 GC/TNAGC Bpu1102I, Bsp1720I, CelII, EspI
BlsI GCN/GC Fnu4HI^ R0178 GC/NGC BisI ^, BthCI^, Fnu4HI^, Fsp4HI^, GluI^, ItaI ^, SatI^
BmcAI AGT/ACT ScaI^ (1) (2) R0122 AGT/ACT Acc113I ^, AssI ^, ScaI^, ZrmI^
Bme1390I CC/NGG BssKI^ R0592 /CCNGG BmrFI, BssKI^, BstSCI^, MspR9I, ScrFI, StyD4I^
ScrFI R0110 CC/NGG
StyD4I^ R0638 /CCNGG
Bme18I G/GWCC AvaII R0153 G/GWCC AflI , AvaII, Eco47I, PspO3I^, SinI, Vpak11AI^
BmeRI GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AhdI, AspEI , DriI, Eam1105I, EclHKI, NruGI
BmeT110I C/YCGRG AvaI R0152 C/YCGRG Ama87I , AvaI, BcoI , BsiHKCI, BsoBI, Eco88I, NspIII
BsoBI R0586 C/YCGRG
BmgBI CACGTC(-3/-3) BmgBI R0628 CACGTC(-3/-3) AjiI , BtrI^
BmgIx GKGCCC
BmgT120I GG/NCC Sau96I^ R0165 G/GNCC AspS9I ^, AsuI ^, BsiZI^, BspLI^, Cfr13I^, FmuI^, PspPI ^, Sau96I^, UnbI^
BmiI GGN/NCC NlaIV R0126 GGN/NCC BscBI, NlaIV, PspN4I
BmrFI CC/NGG BssKI^ R0592 /CCNGG Bme1390I, BssKI^, BstSCI^, MspR9I, ScrFI, StyD4I^
ScrFI R0110 CC/NGG
StyD4I^ R0638 /CCNGG
BmrI ACTGGG(5/4) BmrI R0600 ACTGGG(5/4) BfiI , BmuI
BmsI GCATC(5/9) SfaNI R0172 GCATC(5/9) LweI, SfaNI
BmtI GCTAG/C BmtI (1) R0658 GCTAG/C AsuNHI ^, BspOI, NheI^
NheI^ (1) (2) R0131 G/CTAGC
BmuI ACTGGG(5/4) BmrI R0600 ACTGGG(5/4) BfiI , BmrI
BmyI GDGCH/C Bsp1286I R0120 GDGCH/C Bsp1286I, MhlI, SduI
BoxI GACNN/NNGTC PshAI R0593 GACNN/NNGTC BstPAI, PshAI
BpcI C/TRYAG SfcI R0561 C/TRYAG BfmI , BstSFI, SfcI
BpiI GAAGAC(2/6) BbsI R0539 GAAGAC(2/6) BbsI, BbvII , BpuAI, BstV2I
BplI (8/13)GAGNNNNNCTC(13/8)
BpmI CTGGAG(16/14) BpmI R0565 CTGGAG(16/14) GsuI
BptI CC/WGG BstNI R0168 CC/WGG AjnI ^, BciT130I, BseBI, BsiLI, Bst2UI, BstNI, BstOI, EcoRII^, MvaI, Psp6I ^, PspGI^
PspGI^ R0611 /CCWGG
Bpu10I CCTNAGC(-5/-2) Bpu10I R0649 CCTNAGC(-5/-2)
Bpu1102I GC/TNAGC BlpI R0585 GC/TNAGC BlpI, Bsp1720I, CelII, EspI
Bpu14I TT/CGAA BstBI R0519 TT/CGAA AsuII , BsiCI, Bsp119I, BspT104I, BstBI, Csp45I, NspV , SfuI
BpuAI GAAGAC(2/6) BbsI R0539 GAAGAC(2/6) BbsI, BbvII , BpiI, BstV2I
BpuEI CTTGAG(16/14) BpuEI R0633 CTTGAG(16/14) Bce83I
BpuMI CC/SGG NciI R0196 CC/SGG AsuC2I , BcnI , CauII, EcoHI^, NciI
BpvUIx CGAT/CG PvuI (1) R0150 CGAT/CG BspCI, MvrI, Ple19I , PvuI
Bsa29I AT/CGAT BspDI R0557 AT/CGAT BanIII , BseCI, BshVI, BsiXI, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI
ClaI R0197 AT/CGAT
BsaAI YAC/GTR BsaAI R0531 YAC/GTR BstBAI, Ppu21I
BsaBI GATNN/NNATC BsaBI R0537 GATNN/NNATC Bse8I, BseJI, BsiBI, BsrBRI, MamI
BsaHI GR/CGYC BsaHI R0556 GR/CGYC AcyI , BssNI, BstACI, Hin1I, Hsp92I
BsaI GGTCTC(1/5) BsaI (1) R0535 GGTCTC(1/5) Bso31I, BspTNI, Eco31I
BsaJI C/CNNGG BsaJI R0536 C/CNNGG BseDI, BssECI, SecI
BsaMI GAATGC(1/-1) BsmI R0134 GAATGC(1/-1) BscCI, BsmI, Mva1269I, Nb.BsmI^, PctI
Nb.BsmI^ R0706 GAATGC
BsaOI CGRY/CG BsiEI R0554 CGRY/CG Bsh1285I , BsiEI, BstMCI, McrI
BsaWI W/CCGGW BsaWI R0567 W/CCGGW BetI
BsaXI (9/12)ACNNNNNCTCC(10/7) BsaXI R0609 (9/12)ACNNNNNCTCC(10/7)
BsbIx CAACAC(21/19)
Bsc4I CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG AfiI , BseLI, BsiYI, BslI
BscBI GGN/NCC NlaIV R0126 GGN/NCC BmiI, NlaIV, PspN4I
BscCI GAATGC(1/-1) BsmI R0134 GAATGC(1/-1) BsaMI, BsmI, Mva1269I, Nb.BsmI^, PctI
Nb.BsmI^ R0706 GAATGC
BscFI /GATC BfuCI R0636 /GATC BfuCI, Bsp143I, BssMI, BstENII, BstKTI^, BstMBI, DpnI^, Kzo9I, MalI^, MboI, NdeII^, Sau3AI
DpnI^ R0176 GA/TC
MboI R0147 /GATC
Sau3AI R0169 /GATC
BscGIx CCCGT
Bse118I R/CCGGY BsrFI R0562 R/CCGGY BsrFI, BssAI, Cfr10I
Bse1I ACTGG(1/-1) BsrI R0527 ACTGG(1/-1) BseNI, BsrI
Bse21I CC/TNAGG Bsu36I R0524 CC/TNAGG AocI , AxyI , Bsu36I, CvnI, Eco81I, SauI
Bse3DI GCAATG(2/0) BsrDI R0574 GCAATG(2/0) BseMI, BsrDI, Nb.BsrDI^
Nb.BsrDI^ R0648 GCAATG
Bse8I GATNN/NNATC BsaBI R0537 GATNN/NNATC BsaBI, BseJI, BsiBI, BsrBRI, MamI
BseAI T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI , BlfI , BsiMI, Bsp13I, BspEI, BspMII, Kpn2I , MroI
BseBI CC/WGG BstNI R0168 CC/WGG AjnI ^, BciT130I, BptI, BsiLI, Bst2UI, BstNI, BstOI, EcoRII^, MvaI, Psp6I ^, PspGI^
PspGI^ R0611 /CCWGG
BseCI AT/CGAT BspDI R0557 AT/CGAT BanIII , Bsa29I, BshVI, BsiXI, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI
ClaI R0197 AT/CGAT
BseDI C/CNNGG BsaJI R0536 C/CNNGG BsaJI, BssECI, SecI
BseGI GGATG(2/0) BtsCI R0647 GGATG(2/0) BstF5I, BtsCI, FokI^
FokI^ R0109 GGATG(9/13)
BseJI GATNN/NNATC BsaBI R0537 GATNN/NNATC BsaBI, Bse8I, BsiBI, BsrBRI, MamI
BseLI CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG AfiI , Bsc4I, BsiYI, BslI
BseMI GCAATG(2/0) BsrDI R0574 GCAATG(2/0) Bse3DI, BsrDI, Nb.BsrDI^
Nb.BsrDI^ R0648 GCAATG
BseMII CTCAG(10/8) BspCNI^ R0624 CTCAG(9/7) BspCNI^
BseNI ACTGG(1/-1) BsrI R0527 ACTGG(1/-1) Bse1I, BsrI
BsePI G/CGCGC BssHII R0199 G/CGCGC BssHII, PauI , PteI
BseRI GAGGAG(10/8) BseRI R0581 GAGGAG(10/8)
BseSI GKGCM/C BaeGI R0708 GKGCM/C BaeGI, BstSLI
BseX3I C/GGCCG EagI (1) R0505 C/GGCCG BstZI, EagI, EclXI, Eco52I, XmaIII
BseXI GCAGC(8/12) BbvI R0173 GCAGC(8/12) BbvI, Bst71I, BstV1I, R9896
BseYI CCCAGC(-5/-1) BseYI R0635 CCCAGC(-5/-1) GsaI^
BsgI GTGCAG(16/14) BsgI R0559 GTGCAG(16/14)
Bsh1236I CG/CG BstUI R0518 CG/CG AccII , BspFNI^, BstFNI, BstUI, FnuDII^, MvnI, ThaI
Bsh1285I CGRY/CG BsiEI R0554 CGRY/CG BsaOI, BsiEI, BstMCI, McrI
BshFI GG/CC HaeIII R0108 GG/CC AoxI^, BsnI, BspANI, BsuRI, HaeIII, PalI , PhoI
PhoI R0705 GG/CC
BshI GG/CC HaeIII R0108 GG/CC AoxI^, BshFI , BsnI, BspANI, BsuRI, HaeIII, PalI , PhoI
PhoI R0705 GG/CC
BshNI G/GYRCC BanI R0118 G/GYRCC AccB1I , BanI, BspT107I, HgiCI
BshTI A/CCGGT AgeI (1) (2) R0552 A/CCGGT AgeI, AsiAI , CspAI, PinAI
BshVI AT/CGAT BspDI R0557 AT/CGAT BanIII , Bsa29I, BseCI, BsiXI, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI
ClaI R0197 AT/CGAT
BsiBI GATNN/NNATC BsaBI R0537 GATNN/NNATC BsaBI, Bse8I, BseJI, BsrBRI, MamI
BsiCI TT/CGAA BstBI R0519 TT/CGAA AsuII , Bpu14I, Bsp119I, BspT104I, BstBI, Csp45I, NspV , SfuI
BsiEI CGRY/CG BsiEI R0554 CGRY/CG BsaOI, Bsh1285I , BstMCI, McrI
BsiHKAI GWGCW/C BsiHKAI R0570 GWGCW/C Alw21I , AspHI , Bbv12I , HgiAI
BsiHKCI C/YCGRG AvaI R0152 C/YCGRG Ama87I , AvaI, BcoI , BmeT110I, BsoBI, Eco88I, NspIII
BsoBI R0586 C/YCGRG
BsiIx CACGAG(-5/-1) BssSI R0587 CACGAG(-5/-1) BauI , BssSI, Bst2BI
BsiLI CC/WGG BstNI R0168 CC/WGG AjnI ^, BciT130I, BptI, BseBI, Bst2UI, BstNI, BstOI, EcoRII^, MvaI, Psp6I ^, PspGI^
PspGI^ R0611 /CCWGG
BsiMI T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI , BlfI , BseAI, Bsp13I, BspEI, BspMII, Kpn2I , MroI
BsiQI T/GATCA BclI R0160 T/GATCA BclI, FbaI, Ksp22I
BsiSI C/CGG HpaII R0171 C/CGG HapII, HpaII, MspI
MspI R0106 C/CGG
BsiWI C/GTACG BsiWI R0553 C/GTACG Pfl23II , PspLI , SplI, SunI
BsiXI AT/CGAT BspDI R0557 AT/CGAT BanIII , Bsa29I, BseCI, BshVI, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI
ClaI R0197 AT/CGAT
BsiYIx CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG AfiI , Bsc4I, BseLI, BslI
BsiZI G/GNCC Sau96I R0165 G/GNCC AspS9I , AsuI , BmgT120I^, BspLI^, Cfr13I, FmuI^, PspPI , Sau96I, UnbI^
BslFI GGGAC(10/14) BsmFI R0572 GGGAC(10/14) BsmFI, FaqI, FinI^
BslI CCNNNNN/NNGG BslI R0555 CCNNNNN/NNGG AfiI , Bsc4I, BseLI, BsiYI
BsmAI GTCTC(1/5) BsmAI R0529 GTCTC(1/5) Alw26I , BcoDI, BsoMAI, BstMAI, Nt.BsmAI^
BcoDI R0542 GTCTC(1/5)
Nt.BsmAI^ R0121 GTCTC(1/-5)
BsmBI CGTCTC(1/5) BsmBI R0580 CGTCTC(1/5) Esp3I
BsmFI GGGAC(10/14) BsmFI R0572 GGGAC(10/14) BslFI, FaqI, FinI^
BsmI GAATGC(1/-1) BsmI R0134 GAATGC(1/-1) BsaMI, BscCI, Mva1269I, Nb.BsmI^, PctI
Nb.BsmI^ R0706 GAATGC
BsnI GG/CC HaeIII R0108 GG/CC AoxI^, BshFI , BspANI, BsuRI, HaeIII, PalI , PhoI
PhoI R0705 GG/CC
Bso31I GGTCTC(1/5) BsaI (1) R0535 GGTCTC(1/5) BsaI, BspTNI, Eco31I
BsoBI C/YCGRG BsoBI R0586 C/YCGRG Ama87I , AvaI, BcoI , BmeT110I, BsiHKCI, Eco88I, NspIII
AvaI R0152 C/YCGRG
BsoMAI GTCTC(1/5) BcoDI R0542 GTCTC(1/5) Alw26I , BcoDI, BsmAI, BstMAI, Nt.BsmAI^
BsmAI R0529 GTCTC(1/5)
Nt.BsmAI^ R0121 GTCTC(1/-5)
Bsp106I AT/CGAT BspDI R0557 AT/CGAT BanIII , Bsa29I, BseCI, BshVI, BsiXI, BspDI, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI
ClaI R0197 AT/CGAT
Bsp119I TT/CGAA BstBI R0519 TT/CGAA AsuII , Bpu14I, BsiCI, BspT104I, BstBI, Csp45I, NspV , SfuI
Bsp120I G/GGCCC ApaI^ R0114 GGGCC/C ApaI^, PspOMI
PspOMI R0653 G/GGCCC
Bsp1286I GDGCH/C Bsp1286I R0120 GDGCH/C BmyI, MhlI, SduI
Bsp13I T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI , BlfI , BseAI, BsiMI, BspEI, BspMII, Kpn2I , MroI
Bsp1407I T/GTACA BsrGI R0575 T/GTACA AauI , BsrGI, BstAUI, SspBI
Bsp143I /GATC BfuCI R0636 /GATC BfuCI, BscFI, BssMI, BstENII, BstKTI^, BstMBI, DpnI^, Kzo9I, MalI^, MboI, NdeII^, Sau3AI
DpnI^ R0176 GA/TC
MboI R0147 /GATC
Sau3AI R0169 /GATC
Bsp143II RGCGC/Y HaeII R0107 RGCGC/Y BfoI, BstH2I, HaeII
Bsp1720I GC/TNAGC BlpI R0585 GC/TNAGC BlpI, Bpu1102I, CelII, EspI
Bsp19I C/CATGG NcoI (1) (2) R0193 C/CATGG NcoI
Bsp24Ix (8/13)GACNNNNNNTGG(12/7)
Bsp68I TCG/CGA NruI R0192 TCG/CGA BtuMI, NruI, RruI^
BspA2I C/CTAGG AvrII R0174 C/CTAGG AspA2I , AvrII, BlnI , XmaJI
BspACI CCGC(-3/-1) AciI R0551 CCGC(-3/-1) AciI, SsiI
BspANI GG/CC HaeIII R0108 GG/CC AoxI^, BshFI , BsnI, BsuRI, HaeIII, PalI , PhoI
PhoI R0705 GG/CC
BspCI CGAT/CG PvuI (1) R0150 CGAT/CG BpvUI, MvrI, Ple19I , PvuI
BspCNI CTCAG(9/7) BspCNI R0624 CTCAG(9/7) BseMII^
BspDI AT/CGAT BspDI R0557 AT/CGAT BanIII , Bsa29I, BseCI, BshVI, BsiXI, Bsp106I, BspXI, Bsu15I, BsuTUI, ClaI, ZhoI
ClaI R0197 AT/CGAT
BspEI T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI , BlfI , BseAI, BsiMI, Bsp13I, BspMII, Kpn2I , MroI
BspFNI CG/CG BstUI^ R0518 CG/CG AccII ^, Bsh1236I^, BstFNI^, BstUI^, FnuDII, MvnI^, ThaI^
BspGIx CTGGAC
BspHI T/CATGA BspHI R0517 T/CATGA CciI, PagI , RcaI
BspLI GGN/CC Sau96I^ R0165 G/GNCC AspS9I ^, AsuI ^, BmgT120I^, BsiZI^, Cfr13I^, FmuI^, PspPI ^, Sau96I^, UnbI^
BspLU11Ix A/CATGT PciI R0655 A/CATGT PciI, PscI
BspMAI CTGCA/G PstI (1) R0140 CTGCA/G PstI
BspMI ACCTGC(4/8) BspMI R0502 ACCTGC(4/8) Acc36I , BfuAI, BveI
BfuAI R0701 ACCTGC(4/8)
BspMIIx T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI , BlfI , BseAI, BsiMI, Bsp13I, BspEI, Kpn2I , MroI
BspNCIx CCAGA
BspOI GCTAG/C BmtI (1) R0658 GCTAG/C AsuNHI ^, BmtI, NheI^
NheI^ (1) (2) R0131 G/CTAGC
BspPI GGATC(4/5) AlwI R0513 GGATC(4/5) AclWI , AlwI, BinI, Nt.AlwI^
Nt.AlwI^ R0627 GGATC(4/-5)
BspQI GCTCTTC(1/4) BspQI R0712 GCTCTTC(1/4) LguI, Nt.BspQI^, PciSI , SapI
Nt.BspQI^ R0644 GCTCTTC(1/-7)
SapI R0569 GCTCTTC(1/4)
BspT104I TT/CGAA BstBI R0519 TT/CGAA AsuII , Bpu14I, BsiCI, Bsp119I, BstBI, Csp45I, NspV , SfuI
BspT107I G/GYRCC BanI R0118 G/GYRCC AccB1I , BanI, BshNI, HgiCI
BspTI C/TTAAG AflII R0520 C/TTAAG AflII, BfrI , Bst98I, BstAFI, MspCI, Vha464I
BspTNI GGTCTC(1/5) BsaI (1) R0535 GGTCTC(1/5) BsaI, Bso31I, Eco31I
BspUIx GCSG/C
BspXI AT/CGAT BspDI R0557 AT/CGAT BanIII , Bsa29I, BseCI, BshVI, BsiXI, Bsp106I, BspDI, Bsu15I, BsuTUI, ClaI, ZhoI
ClaI R0197 AT/CGAT
BsrBI CCGCTC(-3/-3) BsrBI R0102 CCGCTC(-3/-3) AccBSI , MbiI
BsrBRI GATNN/NNATC BsaBI R0537 GATNN/NNATC BsaBI, Bse8I, BseJI, BsiBI, MamI
BsrDI GCAATG(2/0) BsrDI R0574 GCAATG(2/0) Bse3DI, BseMI, Nb.BsrDI^
Nb.BsrDI^ R0648 GCAATG
BsrFI R/CCGGY BsrFI R0562 R/CCGGY Bse118I, BssAI, Cfr10I
BsrGI T/GTACA BsrGI R0575 T/GTACA AauI , Bsp1407I, BstAUI, SspBI
BsrI ACTGG(1/-1) BsrI R0527 ACTGG(1/-1) Bse1I, BseNI
BsrSI ACTGG(1/-1) BsrI R0527 ACTGG(1/-1) Bse1I, BseNI, BsrI
BssAI R/CCGGY BsrFI R0562 R/CCGGY Bse118I, BsrFI, Cfr10I
BssECI C/CNNGG BsaJI R0536 C/CNNGG BsaJI, BseDI, SecI
BssHI C/TCGAG PaeR7I R0177 C/TCGAG PaeR7I, Sfr274I, SlaI, StrI, TliI, XhoI
TliI R0612 C/TCGAG
XhoI (2) R0146 C/TCGAG
BssHII G/CGCGC BssHII R0199 G/CGCGC BsePI, PauI , PteI
BssKI /CCNGG BssKI R0592 /CCNGG Bme1390I^, BmrFI^, BstSCI, MspR9I^, ScrFI^, StyD4I
ScrFI^ R0110 CC/NGG
StyD4I R0638 /CCNGG
BssMI /GATC BfuCI R0636 /GATC BfuCI, BscFI, Bsp143I, BstENII, BstKTI^, BstMBI, DpnI^, Kzo9I, MalI^, MboI, NdeII^, Sau3AI
DpnI^ R0176 GA/TC
MboI R0147 /GATC
Sau3AI R0169 /GATC
BssNAI GTA/TAC BstZ17I R0594 GTA/TAC Bst1107I, BstZ17I, SnaI^
BssNI GR/CGYC BsaHI R0556 GR/CGYC AcyI , BsaHI, BstACI, Hin1I, Hsp92I
BssSI CACGAG(-5/-1) BssSI R0587 CACGAG(-5/-1) BauI , BsiI, Bst2BI
BssT1I C/CWWGG StyI (1) R0500 C/CWWGG Eco130I, EcoT14I, ErhI , StyI
Bst1107I GTA/TAC BstZ17I R0594 GTA/TAC BssNAI, BstZ17I, SnaI^
Bst2BI CACGAG(-5/-1) BssSI R0587 CACGAG(-5/-1) BauI , BsiI, BssSI
Bst2UI CC/WGG BstNI R0168 CC/WGG AjnI ^, BciT130I, BptI, BseBI, BsiLI, BstNI, BstOI, EcoRII^, MvaI, Psp6I ^, PspGI^
PspGI^ R0611 /CCWGG
Bst4CI ACN/GT HpyCH4III R0618 ACN/GT HpyCH4III, TaaI, Tsp4CI
Bst6I CTCTTC(1/4) EarI R0528 CTCTTC(1/4) Eam1104I, EarI, Ksp632I
Bst71Ix GCAGC(8/12) BbvI R0173 GCAGC(8/12) BbvI, BseXI, BstV1I, R9896
Bst98I C/TTAAG AflII R0520 C/TTAAG AflII, BfrI , BspTI, BstAFI, MspCI, Vha464I
BstACI GR/CGYC BsaHI R0556 GR/CGYC AcyI , BsaHI, BssNI, Hin1I, Hsp92I
BstAFI C/TTAAG AflII R0520 C/TTAAG AflII, BfrI , BspTI, Bst98I, MspCI, Vha464I
BstAPI GCANNNN/NTGC BstAPI R0654 GCANNNN/NTGC ApaBI^
BstAUI T/GTACA BsrGI R0575 T/GTACA AauI , Bsp1407I, BsrGI, SspBI
BstBAI YAC/GTR BsaAI R0531 YAC/GTR BsaAI, Ppu21I
BstBI TT/CGAA BstBI R0519 TT/CGAA AsuII , Bpu14I, BsiCI, Bsp119I, BspT104I, Csp45I, NspV , SfuI
BstC8I GCN/NGC Cac8I R0579 GCN/NGC Cac8I
BstDEI C/TNAG DdeI R0175 C/TNAG DdeI, HpyF3I
BstDSI C/CRYGG BtgI R0608 C/CRYGG BtgI, DsaI
BstEII  G/GTNACC BstEII  (1) (2) R0162 G/GTNACC BstPI, Eco065I, Eco91I, EcoO65I, PspEI
BstENI CCTNN/NNNAGG EcoNI R0521 CCTNN/NNNAGG EcoNI, XagI
BstENII /GATC BfuCI R0636 /GATC BfuCI, BscFI, Bsp143I, BssMI, BstKTI^, BstMBI, DpnI^, Kzo9I, MalI^, MboI, NdeII^, Sau3AI
DpnI^ R0176 GA/TC
MboI R0147 /GATC
Sau3AI R0169 /GATC
BstF5I GGATG(2/0) BtsCI R0647 GGATG(2/0) BseGI, BtsCI, FokI^
FokI^ R0109 GGATG(9/13)
BstFNI CG/CG BstUI R0518 CG/CG AccII , Bsh1236I, BspFNI^, BstUI, FnuDII^, MvnI, ThaI
BstH2I RGCGC/Y HaeII R0107 RGCGC/Y BfoI, Bsp143II, HaeII
BstHHI GCG/C HhaI R0139 GCG/C AspLEI , CfoI, GlaI^, HhaI, R9529^, HinP1I^, HspAI ^
HinP1I^ R0124 G/CGC
BstHPI GTT/AAC HpaI R0105 GTT/AAC HpaI, KspAI
BstKTI GAT/C BfuCI^ R0636 /GATC BfuCI^, BscFI^, Bsp143I^, BssMI^, BstENII^, BstMBI^, DpnI^, Kzo9I^, MalI^, MboI^, NdeII^, Sau3AI^
DpnI^ R0176 GA/TC
MboI^ R0147 /GATC
Sau3AI^ R0169 /GATC
BstMAI GTCTC(1/5) BcoDI R0542 GTCTC(1/5) Alw26I , BcoDI, BsmAI, BsoMAI, Nt.BsmAI^
BsmAI R0529 GTCTC(1/5)
Nt.BsmAI^ R0121 GTCTC(1/-5)
BstMBI /GATC BfuCI R0636 /GATC BfuCI, BscFI, Bsp143I, BssMI, BstENII, BstKTI^, DpnI^, Kzo9I, MalI^, MboI, NdeII^, Sau3AI
DpnI^ R0176 GA/TC
MboI R0147 /GATC
Sau3AI R0169 /GATC
BstMCI CGRY/CG BsiEI R0554 CGRY/CG BsaOI, Bsh1285I , BsiEI, McrI
BstMWI GCNNNNN/NNGC MwoI R0573 GCNNNNN/NNGC HpyF10VI, MwoI
BstNI CC/WGG BstNI R0168 CC/WGG AjnI ^, BciT130I, BptI, BseBI, BsiLI, Bst2UI, BstOI, EcoRII^, MvaI, Psp6I ^, PspGI^
PspGI^ R0611 /CCWGG
BstNSI RCATG/Y NspI R0602 RCATG/Y NspI, XceI
BstOI CC/WGG BstNI R0168 CC/WGG AjnI ^, BciT130I, BptI, BseBI, BsiLI, Bst2UI, BstNI, EcoRII^, MvaI, Psp6I ^, PspGI^
PspGI^ R0611 /CCWGG
BstPAI GACNN/NNGTC PshAI R0593 GACNN/NNGTC BoxI, PshAI
BstPI G/GTNACC BstEII  (1) (2) R0162 G/GTNACC BstEII , Eco065I, Eco91I, EcoO65I, PspEI
BstSCI /CCNGG BssKI R0592 /CCNGG Bme1390I^, BmrFI^, BssKI, MspR9I^, ScrFI^, StyD4I
ScrFI^ R0110 CC/NGG
StyD4I R0638 /CCNGG
BstSFI C/TRYAG SfcI R0561 C/TRYAG BfmI , BpcI, SfcI
BstSLI GKGCM/C BaeGI R0708 GKGCM/C BaeGI, BseSI
BstSNI TAC/GTA SnaBI R0130 TAC/GTA Eco105I, SnaBI
BstUI CG/CG BstUI R0518 CG/CG AccII , Bsh1236I, BspFNI^, BstFNI, FnuDII^, MvnI, ThaI
BstV1I GCAGC(8/12) BbvI R0173 GCAGC(8/12) BbvI, BseXI, Bst71I, R9896
BstV2I GAAGAC(2/6) BbsI R0539 GAAGAC(2/6) BbsI, BbvII , BpiI, BpuAI
BstX2I R/GATCY BstYI R0523 R/GATCY BstYI, MflI, PsuI, XhoII
BstXI CCANNNNN/NTGG BstXI R0113 CCANNNNN/NTGG
BstYI R/GATCY BstYI R0523 R/GATCY BstX2I, MflI, PsuI, XhoII
BstZ17I GTA/TAC BstZ17I R0594 GTA/TAC BssNAI, Bst1107I, SnaI^
BstZI C/GGCCG EagI (1) R0505 C/GGCCG BseX3I , EagI, EclXI, Eco52I, XmaIII
Bsu15I AT/CGAT BspDI R0557 AT/CGAT BanIII , Bsa29I, BseCI, BshVI, BsiXI, Bsp106I, BspDI, BspXI, BsuTUI, ClaI, ZhoI
ClaI R0197 AT/CGAT
Bsu36I CC/TNAGG Bsu36I R0524 CC/TNAGG AocI , AxyI , Bse21I, CvnI, Eco81I, SauI
BsuI GTATCC(6/5) BciVI R0596 GTATCC(6/5) BciVI, BfuI
BsuRI GG/CC HaeIII R0108 GG/CC AoxI^, BshFI , BsnI, BspANI, HaeIII, PalI , PhoI
PhoI R0705 GG/CC
BsuTUI AT/CGAT BspDI R0557 AT/CGAT BanIII , Bsa29I, BseCI, BshVI, BsiXI, Bsp106I, BspDI, BspXI, Bsu15I, ClaI, ZhoI
ClaI R0197 AT/CGAT
BtgI C/CRYGG BtgI R0608 C/CRYGG BstDSI, DsaI
BtgZI GCGATG(10/14) BtgZI R0703 GCGATG(10/14)
BthCIx GCNG/C Fnu4HI^ R0178 GC/NGC BisI ^, BlsI ^, Fnu4HI^, Fsp4HI^, GluI^, ItaI ^, SatI^
BtrI CACGTC(-3/-3) BmgBI^ R0628 CACGTC(-3/-3) AjiI ^, BmgBI^
BtsCI GGATG(2/0) BtsCI R0647 GGATG(2/0) BseGI, BstF5I, FokI^
FokI^ R0109 GGATG(9/13)
BtsI GCAGTG(2/0) BtsI R0614 GCAGTG(2/0) Nb.BtsI^
Nb.BtsI^ R0707 GCAGTG
BtsIMutI CAGTG(2/0) BtsIMutI R0664 CAGTG(2/0)
BtuMI TCG/CGA NruI R0192 TCG/CGA Bsp68I, NruI, RruI^
BveI ACCTGC(4/8) BfuAI R0701 ACCTGC(4/8) Acc36I , BfuAI, BspMI
BspMI R0502 ACCTGC(4/8)
Cac8I GCN/NGC Cac8I R0579 GCN/NGC BstC8I
CaiI CAGNNN/CTG AlwNI R0514 CAGNNN/CTG AlwNI, PstNI
CauIIx CC/SGG NciI R0196 CC/SGG AsuC2I , BcnI , BpuMI, EcoHI^, NciI
CchIIx GGARGA(11/9)
CchIIIx CCCAAG(20/18)
CciI T/CATGA BspHI R0517 T/CATGA BspHI, PagI , RcaI
CciNI GC/GGCCGC NotI (1) (2) R0189 GC/GGCCGC NotI
CcrNAIII CGACCAG
CdiIx CATCG(-1/-1)
CdpI GCGGAG(20/18)
CelII GC/TNAGC BlpI R0585 GC/TNAGC BlpI, Bpu1102I, Bsp1720I, EspI
CfoI GCG/C HhaI R0139 GCG/C AspLEI , BstHHI, GlaI^, HhaI, R9529^, HinP1I^, HspAI ^
HinP1I^ R0124 G/CGC
Cfr10I R/CCGGY BsrFI R0562 R/CCGGY Bse118I, BsrFI, BssAI
Cfr13I G/GNCC Sau96I R0165 G/GNCC AspS9I , AsuI , BmgT120I^, BsiZI, BspLI^, FmuI^, PspPI , Sau96I, UnbI^
Cfr42I CCGC/GG SacII R0157 CCGC/GG KspI, SacII, Sfr303I, SgrBI, SstII
Cfr9I C/CCGGG SmaI^ R0141 CCC/GGG PspAI , SmaI^, TspMI, XmaCI
TspMI R0709 C/CCGGG
CfrIx Y/GGCCR EaeI R0508 Y/GGCCR AcoI , EaeI
Cgl13032I GGCGCA
Cgl13032II ACGABGG
CjeFIIIx GCAAGG
CjeFVx GGRCA
CjeIx (8/14)CCANNNNNNGT(15/9)
CjeNIIx GAGNNNNNGT
CjeNIIIx GKAAYG(19/17)
CjeP659IVx CACNNNNNNNGAA
CjePIx (7/13)CCANNNNNNNTC(14/8)
CjuIx CAYNNNNNRTG
CjuIIx CAYNNNNNCTC
ClaI AT/CGAT ClaI R0197 AT/CGAT BanIII , Bsa29I, BseCI, BshVI, BsiXI, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ZhoI
BspDI R0557 AT/CGAT
Cly7489II AAAACRG
CpoI CG/GWCCG
CseI GACGC(5/10) HgaI R0154 GACGC(5/10) HgaI
CsiI A/CCWGGT SexAI R0605 A/CCWGGT MabI, SexAI
Csp45I TT/CGAA BstBI R0519 TT/CGAA AsuII , Bpu14I, BsiCI, Bsp119I, BspT104I, BstBI, NspV , SfuI
Csp6I G/TAC CviQI R0639 G/TAC AfaI ^, CviQI, RsaI^
RsaI^ R0167 GT/AC
CspAI A/CCGGT AgeI (1) (2) R0552 A/CCGGT AgeI, AsiAI , BshTI, PinAI
CspCI (11/13)CAANNNNNGTGG(12/10) CspCI R0645 (11/13)CAANNNNNGTGG(12/10)
CspI CG/GWCCG
CstMIx AAGGAG(20/18)
CviAII C/ATG CviAII R0640 C/ATG FaeI^, FatI^, Hin1II^, Hsp92II ^, NlaIII^
FatI^ R0650 /CATG
NlaIII^ R0125 CATG/
CviJI RG/CY
CviKI-1 RG/CY CviKI-1 R0710 RG/CY CviJI^, CviTI^
CviQI G/TAC CviQI R0639 G/TAC AfaI ^, Csp6I, RsaI^
RsaI^ R0167 GT/AC
CviRIx TG/CA HpyCH4V R0620 TG/CA HpyCH4V
CviTI RG/CY
CvnI CC/TNAGG Bsu36I R0524 CC/TNAGG AocI , AxyI , Bse21I, Bsu36I, Eco81I, SauI
DdeI C/TNAG DdeI R0175 C/TNAG BstDEI, HpyF3I
DinI GGC/GCC KasI^ R0544 G/GCGCC BbeI ^, EgeI, KasI^, Mly113I^, NarI^, PluTI^, SfoI, SspDI^
NarI^ R0191 GG/CGCC
PluTI^ R0713 GGCGC/C
SfoI R0606 GGC/GCC
DpnI GA/TC DpnI R0176 GA/TC BfuCI^, BscFI^, Bsp143I^, BssMI^, BstENII^, BstKTI^, BstMBI^, Kzo9I^, MalI^, MboI^, NdeII^, Sau3AI^
BfuCI^ R0636 /GATC
MboI^ R0147 /GATC
Sau3AI^ R0169 /GATC
DpnII /GATC DpnII R0543 /GATC BfuCI, BscFI, Bsp143I, BssMI, BstENII, BstKTI^, BstMBI, DpnI^, Kzo9I, MalI^, MboI, NdeII^, Sau3AI
DpnI^ R0176 GA/TC
MboI R0147 /GATC
Sau3AI R0169 /GATC
DraI TTT/AAA DraI R0129 TTT/AAA AhaIII ^
DraII RG/GNCCY EcoO109I R0503 RG/GNCCY EcoO109I, PssI^
DraIII CACNNN/GTG DraIII (1) R0510 CACNNN/GTG AdeI
DraRIx CAAGNAC(20/18)
DrdI GACNNNN/NNGTC DrdI R0530 GACNNNN/NNGTC AasI , DseDI
DrdIIx GAACCA
DriI GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AhdI, AspEI , BmeRI, Eam1105I, EclHKI, NruGI
DsaIx C/CRYGG BtgI R0608 C/CRYGG BstDSI, BtgI
DseDI GACNNNN/NNGTC DrdI R0530 GACNNNN/NNGTC AasI , DrdI
DvuIIIx CACNCAC
EaeI Y/GGCCR EaeI R0508 Y/GGCCR AcoI , CfrI
EagI C/GGCCG EagI (1) R0505 C/GGCCG BseX3I , BstZI, EclXI, Eco52I, XmaIII
Eam1104I CTCTTC(1/4) EarI R0528 CTCTTC(1/4) Bst6I, EarI, Ksp632I
Eam1105I GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AhdI, AspEI , BmeRI, DriI, EclHKI, NruGI
EarI CTCTTC(1/4) EarI R0528 CTCTTC(1/4) Bst6I, Eam1104I, Ksp632I
EciI GGCGGA(11/9) EciI R0590 GGCGGA(11/9)
Ecl136II GAG/CTC Eco53kI R0116 GAG/CTC Eco53kI, EcoICRI, Psp124BI ^, SacI^, SstI^
SacI^ (1) R0156 GAGCT/C
EclHKI GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AhdI, AspEI , BmeRI, DriI, Eam1105I, NruGI
EclXI C/GGCCG EagI (1) R0505 C/GGCCG BseX3I , BstZI, EagI, Eco52I, XmaIII
Eco065I G/GTNACC BstEII  (1) (2) R0162 G/GTNACC BstEII , BstPI, Eco91I, EcoO65I, PspEI
Eco105I TAC/GTA SnaBI R0130 TAC/GTA BstSNI, SnaBI
Eco130I C/CWWGG StyI (1) R0500 C/CWWGG BssT1I, EcoT14I, ErhI , StyI
Eco147I AGG/CCT StuI R0187 AGG/CCT AatI , PceI , SseBI, StuI
Eco24I GRGCY/C BanII R0119 GRGCY/C BanII, EcoT38I, FriOI, HgiJII
Eco31I GGTCTC(1/5) BsaI (1) R0535 GGTCTC(1/5) BsaI, Bso31I, BspTNI
Eco32I GAT/ATC EcoRV (1) (2) R0195 GAT/ATC EcoRV
Eco47I G/GWCC AvaII R0153 G/GWCC AflI , AvaII, Bme18I, PspO3I^, SinI, Vpak11AI^
Eco47III AGC/GCT AfeI R0652 AGC/GCT AfeI, Aor51HI , FunI
Eco52I C/GGCCG EagI (1) R0505 C/GGCCG BseX3I , BstZI, EagI, EclXI, XmaIII
Eco53kI GAG/CTC Eco53kI R0116 GAG/CTC Ecl136II, EcoICRI, Psp124BI ^, SacI^, SstI^
SacI^ (1) R0156 GAGCT/C
Eco57I CTGAAG(16/14) AcuI R0641 CTGAAG(16/14) AcuI
Eco57MIx CTGRAG(16/14)
Eco72I CAC/GTG PmlI R0532 CAC/GTG AcvI , BbrPI , PmaCI , PmlI, PspCI
Eco81I CC/TNAGG Bsu36I R0524 CC/TNAGG AocI , AxyI , Bse21I, Bsu36I, CvnI, SauI
Eco88I C/YCGRG AvaI R0152 C/YCGRG Ama87I , AvaI, BcoI , BmeT110I, BsiHKCI, BsoBI, NspIII
BsoBI R0586 C/YCGRG
Eco91I G/GTNACC BstEII  (1) (2) R0162 G/GTNACC BstEII , BstPI, Eco065I, EcoO65I, PspEI
EcoHIx /CCSGG NciI^ R0196 CC/SGG AsuC2I ^, BcnI ^, BpuMI^, CauII^, NciI^
EcoICRI GAG/CTC Eco53kI R0116 GAG/CTC Ecl136II, Eco53kI, Psp124BI ^, SacI^, SstI^
SacI^ (1) R0156 GAGCT/C
EcoNI CCTNN/NNNAGG EcoNI R0521 CCTNN/NNNAGG BstENI, XagI
EcoO109I RG/GNCCY EcoO109I R0503 RG/GNCCY DraII, PssI^
EcoO65I G/GTNACC BstEII  (1) (2) R0162 G/GTNACC BstEII , BstPI, Eco065I, Eco91I, PspEI
EcoP15I CAGCAG(25/27) EcoP15I R0646 CAGCAG(25/27)
EcoRI G/AATTC EcoRI (1) (2) R0101 G/AATTC FunII
EcoRII /CCWGG BstNI^ R0168 CC/WGG AjnI ^, BciT130I^, BptI^, BseBI^, BsiLI^, Bst2UI^, BstNI^, BstOI^, MvaI^, Psp6I , PspGI
PspGI R0611 /CCWGG
EcoRV GAT/ATC EcoRV (1) (2) R0195 GAT/ATC Eco32I
EcoT14I C/CWWGG StyI (1) R0500 C/CWWGG BssT1I, Eco130I, ErhI , StyI
EcoT22I ATGCA/T NsiI R0127 ATGCA/T AvaIII ^, BfrBl, Mph1103I, NsiI, Zsp2I
EcoT38I GRGCY/C BanII R0119 GRGCY/C BanII, Eco24I, FriOI, HgiJII
EgeI GGC/GCC KasI^ R0544 G/GCGCC BbeI ^, DinI, KasI^, Mly113I^, NarI^, PluTI^, SfoI, SspDI^
NarI^ R0191 GG/CGCC
PluTI^ R0713 GGCGC/C
SfoI R0606 GGC/GCC
EheI GGC/GCC KasI^ R0544 G/GCGCC BbeI ^, DinI, EgeI, KasI^, Mly113I^, NarI^, PluTI^, SfoI, SspDI^
NarI^ R0191 GG/CGCC
PluTI^ R0713 GGCGC/C
SfoI R0606 GGC/GCC
ErhI C/CWWGG StyI (1) R0500 C/CWWGG BssT1I, Eco130I, EcoT14I, StyI
EsaSSIx GACCAC
Esp3I CGTCTC(1/5) BsmBI R0580 CGTCTC(1/5) BsmBI
EspIx GC/TNAGC BlpI R0585 GC/TNAGC BlpI, Bpu1102I, Bsp1720I, CelII
FaeI CATG/ CviAII^ R0640 C/ATG CviAII^, FatI^, Hin1II, Hsp92II , NlaIII
FatI^ R0650 /CATG
NlaIII R0125 CATG/
FaiI YA/TR
FalI (8/13)AAGNNNNNCTT(13/8)
FaqI GGGAC(10/14) BsmFI R0572 GGGAC(10/14) BslFI, BsmFI, FinI^
FatI /CATG FatI R0650 /CATG CviAII^, FaeI^, Hin1II^, Hsp92II ^, NlaIII^
CviAII^ R0640 C/ATG
NlaIII^ R0125 CATG/
FauI CCCGC(4/6) FauI R0651 CCCGC(4/6) SmuI
FauNDI CA/TATG NdeI R0111 CA/TATG NdeI
FbaI T/GATCA BclI R0160 T/GATCA BclI, BsiQI, Ksp22I
FblI GT/MKAC AccI R0161 GT/MKAC AccI, XmiI
FinIx GGGAC BsmFI^ R0572 GGGAC(10/14) BslFI^, BsmFI^, FaqI^
FmuIx GGNC/C Sau96I^ R0165 G/GNCC AspS9I ^, AsuI ^, BmgT120I^, BsiZI^, BspLI^, Cfr13I^, PspPI ^, Sau96I^, UnbI^
Fnu4HI GC/NGC Fnu4HI R0178 GC/NGC BisI , BlsI ^, BthCI^, Fsp4HI, GluI, ItaI , SatI
FnuDIIx CG/CG BstUI^ R0518 CG/CG AccII ^, Bsh1236I^, BspFNI, BstFNI^, BstUI^, MvnI^, ThaI^
FokI GGATG(9/13) FokI R0109 GGATG(9/13) BseGI^, BstF5I^, BtsCI^
BtsCI^ R0647 GGATG(2/0)
FriOI GRGCY/C BanII R0119 GRGCY/C BanII, Eco24I, EcoT38I, HgiJII
FseI GGCCGG/CC FseI R0588 GGCCGG/CC RigI
Fsp4HI GC/NGC Fnu4HI R0178 GC/NGC BisI , BlsI ^, BthCI^, Fnu4HI, GluI, ItaI , SatI
FspAI RTGC/GCAY
FspBI C/TAG BfaI R0568 C/TAG BfaI, MaeI, XspI
FspEI CC(12/16) FspEI R0662 CC(12/16)
FspI TGC/GCA FspI R0135 TGC/GCA Acc16I , AviII , MstI, NsbI
FunI AGC/GCT AfeI R0652 AGC/GCT AfeI, Aor51HI , Eco47III
FunII G/AATTC EcoRI (1) (2) R0101 G/AATTC EcoRI
GauT27Ix CGCGCAGG
Gba708II ATGCAC
GdiIIx CGGCCR(-5/-1)
GlaI GC/GC HhaI^ R0139 GCG/C AspLEI ^, BstHHI^, CfoI^, HhaI^, R9529^, HinP1I^, HspAI ^
HinP1I^ R0124 G/CGC
GluI GC/NGC Fnu4HI R0178 GC/NGC BisI , BlsI ^, BthCI^, Fnu4HI, Fsp4HI, ItaI , SatI
GsaI CCCAGC(-1/-5) BseYI^ R0635 CCCAGC(-5/-1) BseYI^
GsuI CTGGAG(16/14) BpmI R0565 CTGGAG(16/14) BpmI
HaeIx WGG/CCW
HaeII RGCGC/Y HaeII R0107 RGCGC/Y BfoI, Bsp143II, BstH2I
HaeIII GG/CC HaeIII R0108 GG/CC AoxI^, BshFI , BsnI, BspANI, BsuRI, PalI , PhoI
PhoI R0705 GG/CC
HaeIVx (7/13)GAYNNNNNRTC(14/9)
HapII C/CGG HpaII R0171 C/CGG BsiSI, HpaII, MspI
MspI R0106 C/CGG
HbaII GCCCAG
HgaI GACGC(5/10) HgaI R0154 GACGC(5/10) CseI
HgiAIx GWGCW/C BsiHKAI R0570 GWGCW/C Alw21I , AspHI , Bbv12I , BsiHKAI
HgiCIx G/GYRCC BanI R0118 G/GYRCC AccB1I , BanI, BshNI, BspT107I
HgiEIIx ACCNNNNNNGGT
HgiJIIx GRGCY/C BanII R0119 GRGCY/C BanII, Eco24I, EcoT38I, FriOI
HhaI GCG/C HhaI R0139 GCG/C AspLEI , BstHHI, CfoI, GlaI^, R9529^, HinP1I^, HspAI ^
HinP1I^ R0124 G/CGC
Hin1I GR/CGYC BsaHI R0556 GR/CGYC AcyI , BsaHI, BssNI, BstACI, Hsp92I
Hin1II CATG/ CviAII^ R0640 C/ATG CviAII^, FaeI, FatI^, Hsp92II , NlaIII
FatI^ R0650 /CATG
NlaIII R0125 CATG/
Hin4Ix (8/13)GAYNNNNNVTC(13/8)
Hin4IIx CCTTC(6/5) HpyAV R0621 CCTTC(6/5) HpyAV
R9529 G/CGC HhaI^ R0139 GCG/C AspLEI ^, BstHHI^, CfoI^, GlaI^, HhaI^, HinP1I, HspAI
HinP1I R0124 G/CGC
Hin6I G/CGC HhaI^ R0139 GCG/C AspLEI ^, BstHHI^, CfoI^, GlaI^, HhaI^, R9529, HinP1I, HspAI
HinP1I R0124 G/CGC
HincII GTY/RAC HincII R0103 GTY/RAC
HindII GTY/RAC
HindIII A/AGCTT HindIII (1) R0104 A/AGCTT
HinfI G/ANTC HinfI R0155 G/ANTC
HinP1I G/CGC HinP1I R0124 G/CGC AspLEI ^, BstHHI^, CfoI^, GlaI^, HhaI^, R9529, HspAI
HhaI^ R0139 GCG/C
HpaI GTT/AAC HpaI R0105 GTT/AAC BstHPI, KspAI
HpaII C/CGG HpaII R0171 C/CGG BsiSI, HapII, MspI
MspI R0106 C/CGG
HphI GGTGA(8/7) HphI R0158 GGTGA(8/7) AsuHPI
Hpy166II GTN/NAC Hpy166II R0616 GTN/NAC Hpy8I^, MjaIV^
Hpy178IIIx TC/NNGA Hpy188III R0622 TC/NNGA Hpy188III
Hpy188I TCN/GA Hpy188I R0617 TCN/GA
Hpy188III TC/NNGA Hpy188III R0622 TC/NNGA Hpy178III
Hpy8I GTN/NAC Hpy166II^ R0616 GTN/NAC Hpy166II^, MjaIV^
Hpy99I CGWCG/ Hpy99I R0615 CGWCG/
Hpy99XIIIx GCCTA
HpyAV CCTTC(6/5) HpyAV R0621 CCTTC(6/5) Hin4II
HpyCH4III ACN/GT HpyCH4III R0618 ACN/GT Bst4CI, TaaI, Tsp4CI
HpyCH4IV A/CGT HpyCH4IV R0619 A/CGT HpySE526I, MaeII, TaiI^, TscI^
HpyCH4V TG/CA HpyCH4V R0620 TG/CA CviRI
HpyF10VI GCNNNNN/NNGC MwoI R0573 GCNNNNN/NNGC BstMWI, MwoI
HpyF3I C/TNAG DdeI R0175 C/TNAG BstDEI, DdeI
HpySE526I A/CGT HpyCH4IV R0619 A/CGT HpyCH4IV, MaeII, TaiI^, TscI^
Hsp92I GR/CGYC BsaHI R0556 GR/CGYC AcyI , BsaHI, BssNI, BstACI, Hin1I
Hsp92II CATG/ CviAII^ R0640 C/ATG CviAII^, FaeI, FatI^, Hin1II, NlaIII
FatI^ R0650 /CATG
NlaIII R0125 CATG/
HspAI G/CGC HhaI^ R0139 GCG/C AspLEI ^, BstHHI^, CfoI^, GlaI^, HhaI^, R9529, HinP1I
HinP1I R0124 G/CGC
I-CeuI TAACTATAACGGTCCTAAGGTAGCGAA(-9/-13) I-CeuI R0699 TAACTATAACGGTCCTAAGGTAGCGAA(-9/-13)
I-SceI TAGGGATAACAGGGTAAT(-9/-13) I-SceI R0694 TAGGGATAACAGGGTAAT(-9/-13)
ItaI GC/NGC Fnu4HI R0178 GC/NGC BisI , BlsI ^, BthCI^, Fnu4HI, Fsp4HI, GluI, SatI
KasI G/GCGCC KasI R0544 G/GCGCC BbeI ^, DinI^, EgeI^, Mly113I^, NarI^, PluTI^, SfoI^, SspDI
NarI^ R0191 GG/CGCC
PluTI^ R0713 GGCGC/C
SfoI^ R0606 GGC/GCC
KflI GG/GWCCC
Kpn2I T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI , BlfI , BseAI, BsiMI, Bsp13I, BspEI, BspMII, MroI
KpnI GGTAC/C KpnI (1) (2) R0142 GGTAC/C Acc65I^, Asp718I ^
Acc65I^ R0599 G/GTACC
KroI G/CCGGC NaeI^ R0190 GCC/GGC MroNI, NaeI^, NgoAIV, NgoMIV, PdiI ^
NgoMIV R0564 G/CCGGC
Ksp22I T/GATCA BclI R0160 T/GATCA BclI, BsiQI, FbaI
Ksp632Ix CTCTTC(1/4) EarI R0528 CTCTTC(1/4) Bst6I, Eam1104I, EarI
Ksp632Ix CTCTTC(1/4) EarI R0528 CTCTTC(1/4) Bst6I, Eam1104I, EarI, Ksp632I
KspAI GTT/AAC HpaI R0105 GTT/AAC BstHPI, HpaI
KspI CCGC/GG SacII R0157 CCGC/GG Cfr42I, SacII, Sfr303I, SgrBI, SstII
Kzo9I /GATC BfuCI R0636 /GATC BfuCI, BscFI, Bsp143I, BssMI, BstENII, BstKTI^, BstMBI, DpnI^, MalI^, MboI, NdeII^, Sau3AI
DpnI^ R0176 GA/TC
MboI R0147 /GATC
Sau3AI R0169 /GATC
LguI GCTCTTC(1/4) BspQI R0712 GCTCTTC(1/4) BspQI, Nt.BspQI^, PciSI , SapI
Nt.BspQI^ R0644 GCTCTTC(1/-7)
SapI R0569 GCTCTTC(1/4)
LlaGIx CTNGAYG
Lmo911II TAGRAG
LpnPI CCDG(10/14) LpnPI R0663 CCDG(10/14)
LweI GCATC(5/9) SfaNI R0172 GCATC(5/9) BmsI, SfaNI
MabI A/CCWGGT SexAI R0605 A/CCWGGT CsiI, SexAI
MaeI C/TAG BfaI R0568 C/TAG BfaI, FspBI, XspI
MaeII A/CGT HpyCH4IV R0619 A/CGT HpyCH4IV, HpySE526I, TaiI^, TscI^
MaeIII /GTNAC
MalI GA/TC BfuCI^ R0636 /GATC BfuCI^, BscFI^, Bsp143I^, BssMI^, BstENII^, BstKTI^, BstMBI^, DpnI^, Kzo9I^, MboI^, NdeII^, Sau3AI^
DpnI^ R0176 GA/TC
MboI^ R0147 /GATC
Sau3AI^ R0169 /GATC
MamI GATNN/NNATC BsaBI R0537 GATNN/NNATC BsaBI, Bse8I, BseJI, BsiBI, BsrBRI
MaqIx CRTTGAC(21/19)
MauBI CG/CGCGCG
MbiI CCGCTC(-3/-3) BsrBI R0102 CCGCTC(-3/-3) AccBSI , BsrBI
MboI /GATC MboI R0147 /GATC BfuCI, BscFI, Bsp143I, BssMI, BstENII, BstKTI^, BstMBI, DpnI^, Kzo9I, MalI^, NdeII^, Sau3AI
BfuCI R0636 /GATC
DpnI^ R0176 GA/TC
Sau3AI R0169 /GATC
MboII GAAGA(8/7) MboII R0148 GAAGA(8/7)
McrIx CGRY/CG BsiEI R0554 CGRY/CG BsaOI, Bsh1285I , BsiEI, BstMCI
MfeI C/AATTG MfeI (1) (2) R0589 C/AATTG MunI
MflI R/GATCY BstYI R0523 R/GATCY BstX2I, BstYI, PsuI, XhoII
MhlI GDGCH/C Bsp1286I R0120 GDGCH/C BmyI, Bsp1286I, SduI
MjaIVx GTNNAC Hpy166II^ R0616 GTN/NAC Hpy166II^, Hpy8I^
MkaDII GAGAYGT
MlsI TGG/CCA MscI R0534 TGG/CCA BalI , MluNI, MscI, Msp20I
MluCI /AATT MluCI R0538 /AATT Sse9I, TasI, Tsp509I, TspEI
Tsp509I R0576 /AATT
MluI A/CGCGT MluI R0198 A/CGCGT
MluNI TGG/CCA MscI R0534 TGG/CCA BalI , MlsI, MscI, Msp20I
Mly113I GG/CGCC KasI^ R0544 G/GCGCC BbeI ^, DinI^, EgeI^, KasI^, NarI, PluTI^, SfoI^, SspDI^
NarI R0191 GG/CGCC
PluTI^ R0713 GGCGC/C
SfoI^ R0606 GGC/GCC
MlyI GAGTC(5/5) MlyI R0610 GAGTC(5/5) Nt.BstNBI^, PleI^, PpsI ^, SchI
Nt.BstNBI^ R0607 GAGTC(4/-5)
PleI^ R0515 GAGTC(4/5)
MmeI TCCRAC(20/18) MmeI R0637 TCCRAC(20/18)
MnlI CCTC(7/6) MnlI R0163 CCTC(7/6)
Mph1103I ATGCA/T NsiI R0127 ATGCA/T AvaIII ^, BfrBl, EcoT22I, NsiI, Zsp2I
MreI CG/CCGGCG
MroI T/CCGGA BspEI R0540 T/CCGGA AccIII, Aor13HI , BlfI , BseAI, BsiMI, Bsp13I, BspEI, BspMII, Kpn2I
MroNI G/CCGGC NaeI^ R0190 GCC/GGC KroI, NaeI^, NgoAIV, NgoMIV, PdiI ^
NgoMIV R0564 G/CCGGC
MroXI GAANN/NNTTC XmnI R0194 GAANN/NNTTC Asp700I , PdmI , XmnI
MscI TGG/CCA MscI R0534 TGG/CCA BalI , MlsI, MluNI, Msp20I
MseI T/TAA MseI R0525 T/TAA SaqAI, Tru1I
MslI CAYNN/NNRTG MslI R0571 CAYNN/NNRTG RseI^, SmiMI
Msp20I TGG/CCA MscI R0534 TGG/CCA BalI , MlsI, MluNI, MscI
MspA1I CMG/CKG MspA1I R0577 CMG/CKG NspBII
MspCI C/TTAAG AflII R0520 C/TTAAG AflII, BfrI , BspTI, Bst98I, BstAFI, Vha464I
MspI C/CGG MspI R0106 C/CGG BsiSI, HapII, HpaII
HpaII R0171 C/CGG
MspJI CNNR(9/13) MspJI R0661 CNNR(9/13)
MspR9I CC/NGG BssKI^ R0592 /CCNGG Bme1390I, BmrFI, BssKI^, BstSCI^, ScrFI, StyD4I^
ScrFI R0110 CC/NGG
StyD4I^ R0638 /CCNGG
MssI GTTT/AAAC PmeI R0560 GTTT/AAAC PmeI
MstIx TGC/GCA FspI R0135 TGC/GCA Acc16I , AviII , FspI, NsbI
MtuHN878II CACGCAG
MunI C/AATTG MfeI (1) (2) R0589 C/AATTG MfeI
Mva1269I GAATGC(1/-1) BsmI R0134 GAATGC(1/-1) BsaMI, BscCI, BsmI, Nb.BsmI^, PctI
Nb.BsmI^ R0706 GAATGC
MvaI CC/WGG BstNI R0168 CC/WGG AjnI ^, BciT130I, BptI, BseBI, BsiLI, Bst2UI, BstNI, BstOI, EcoRII^, Psp6I ^, PspGI^
PspGI^ R0611 /CCWGG
MvnI CG/CG BstUI R0518 CG/CG AccII , Bsh1236I, BspFNI^, BstFNI, BstUI, FnuDII^, ThaI
MvrIx CGAT/CG PvuI (1) R0150 CGAT/CG BpvUI, BspCI, Ple19I , PvuI
MwoI GCNNNNN/NNGC MwoI R0573 GCNNNNN/NNGC BstMWI, HpyF10VI
NaeI GCC/GGC NaeI R0190 GCC/GGC KroI^, MroNI^, NgoAIV^, NgoMIV^, PdiI
NgoMIV^ R0564 G/CCGGC
NarI GG/CGCC NarI R0191 GG/CGCC BbeI ^, DinI^, EgeI^, KasI^, Mly113I, PluTI^, SfoI^, SspDI^
KasI^ R0544 G/GCGCC
PluTI^ R0713 GGCGC/C
SfoI^ R0606 GGC/GCC
Nb.BbvCI CCTCAGC Nb.BbvCI R0631 CCTCAGC BbvCI^, Nt.BbvCI^
BbvCI^ R0601 CCTCAGC(-5/-2)
Nt.BbvCI^ R0632 CCTCAGC(-5/-7)
Nb.BsmI GAATGC Nb.BsmI R0706 GAATGC BsaMI^, BscCI^, BsmI^, Mva1269I^, PctI ^
BsmI^ R0134 GAATGC(1/-1)
Nb.BsrDI GCAATG Nb.BsrDI R0648 GCAATG Bse3DI^, BseMI^, BsrDI^
BsrDI^ R0574 GCAATG(2/0)
Nb.BtsI GCAGTG Nb.BtsI R0707 GCAGTG BtsI^
BtsI^ R0614 GCAGTG(2/0)
NciI CC/SGG NciI R0196 CC/SGG AsuC2I , BcnI , BpuMI, CauII, EcoHI^
NcoI C/CATGG NcoI (1) (2) R0193 C/CATGG Bsp19I
NdeI CA/TATG NdeI R0111 CA/TATG FauNDI
NdeII /GATC BfuCI^ R0636 /GATC BfuCI^, BscFI^, Bsp143I^, BssMI^, BstENII^, BstKTI^, BstMBI^, DpnI^, Kzo9I^, MalI^, MboI^, Sau3AI^
DpnI^ R0176 GA/TC
MboI^ R0147 /GATC
Sau3AI^ R0169 /GATC
NgoAIV G/CCGGC NaeI^ R0190 GCC/GGC KroI, MroNI, NaeI^, NgoMIV, PdiI ^
NgoMIV R0564 G/CCGGC
NgoAVIIIx (12/14)GACNNNNNTGA(13/11)
NgoMIV G/CCGGC NgoMIV R0564 G/CCGGC KroI, MroNI, NaeI^, NgoAIV, PdiI ^
NaeI^ R0190 GCC/GGC
NhaXIx CAAGRAG
NheI G/CTAGC NheI (1) (2) R0131 G/CTAGC AsuNHI , BmtI^, BspOI^
BmtI^ (1) R0658 GCTAG/C
NlaCIx CATCAC(19/17)
NlaIII CATG/ NlaIII R0125 CATG/ CviAII^, FaeI, FatI^, Hin1II, Hsp92II
CviAII^ R0640 C/ATG
FatI^ R0650 /CATG
NlaIV GGN/NCC NlaIV R0126 GGN/NCC BmiI, BscBI, PspN4I
NmeAIII GCCGAG(21/19) NmeAIII R0711 GCCGAG(21/19)
NmuCI /GTSAC Tsp45I R0583 /GTSAC TseFI^, Tsp45I
NotI GC/GGCCGC NotI (1) (2) R0189 GC/GGCCGC CciNI
NruGI GACNNN/NNGTC AhdI R0584 GACNNN/NNGTC AhdI, AspEI , BmeRI, DriI, Eam1105I, EclHKI
NruI TCG/CGA NruI R0192 TCG/CGA Bsp68I, BtuMI, RruI^
NsbI TGC/GCA FspI R0135 TGC/GCA Acc16I , AviII , FspI, MstI
NsiI ATGCA/T NsiI R0127 ATGCA/T AvaIII ^, BfrBl, EcoT22I, Mph1103I, Zsp2I
NspBII x CMG/CKG MspA1I R0577 CMG/CKG MspA1I
NspI RCATG/Y NspI R0602 RCATG/Y BstNSI, XceI
NspIII C/YCGRG AvaI R0152 C/YCGRG Ama87I , AvaI, BcoI , BmeT110I, BsiHKCI, BsoBI, Eco88I
BsoBI R0586 C/YCGRG
NspV TT/CGAA BstBI R0519 TT/CGAA AsuII , Bpu14I, BsiCI, Bsp119I, BspT104I, BstBI, Csp45I, SfuI
Nt.AlwI GGATC(4/-5) Nt.AlwI R0627 GGATC(4/-5) AclWI ^, AlwI^, BinI^, BspPI^
AlwI^ R0513 GGATC(4/5)
Nt.BbvCI CCTCAGC(-5/-7) Nt.BbvCI R0632 CCTCAGC(-5/-7) BbvCI^, Nb.BbvCI^
BbvCI^ R0601 CCTCAGC(-5/-2)
Nb.BbvCI^ R0631 CCTCAGC
Nt.BsmAI GTCTC(1/-5) Nt.BsmAI R0121 GTCTC(1/-5) Alw26I ^, BcoDI^, BsmAI^, BsoMAI^, BstMAI^
BcoDI^ R0542 GTCTC(1/5)
BsmAI^ R0529 GTCTC(1/5)
Nt.BspQI GCTCTTC(1/-7) Nt.BspQI R0644 GCTCTTC(1/-7) BspQI^, LguI^, PciSI ^, SapI^
BspQI^ R0712 GCTCTTC(1/4)
SapI^ R0569 GCTCTTC(1/4)
Nt.BstNBI GAGTC(4/-5) Nt.BstNBI R0607 GAGTC(4/-5) MlyI^, PleI^, PpsI ^, SchI^
MlyI^ R0610 GAGTC(5/5)
PleI^ R0515 GAGTC(4/5)
Nt.CviPII (0/-1)CCD Nt.CviPII R0626 (0/-1)CCD
OliI CACNN/NNGTG AleI R0634 CACNN/NNGTG AleI
PacI TTAAT/TAA PacI (2) R0547 TTAAT/TAA
PaeI GCATG/C SphI (1) R0182 GCATG/C BbuI , SpaHI, SphI
PaeR7I C/TCGAG PaeR7I R0177 C/TCGAG BssHI, Sfr274I, SlaI, StrI, TliI, XhoI
TliI R0612 C/TCGAG
XhoI (2) R0146 C/TCGAG
PagI T/CATGA BspHI R0517 T/CATGA BspHI, CciI, RcaI
PalAI GG/CGCGCC AscI (2) R0558 GG/CGCGCC AscI, SgsI
PalI GG/CC HaeIII R0108 GG/CC AoxI^, BshFI , BsnI, BspANI, BsuRI, HaeIII, PhoI
PhoI R0705 GG/CC
PasI CC/CWGGG
PauI G/CGCGC BssHII R0199 G/CGCGC BsePI, BssHII, PteI
PceI AGG/CCT StuI R0187 AGG/CCT AatI , Eco147I, SseBI, StuI
PciI A/CATGT PciI R0655 A/CATGT BspLU11I, PscI
PciSI GCTCTTC(1/4) BspQI R0712 GCTCTTC(1/4) BspQI, LguI, Nt.BspQI^, SapI
Nt.BspQI^ R0644 GCTCTTC(1/-7)
SapI R0569 GCTCTTC(1/4)
PcsI WCGNNNN/NNNCGW
PctI GAATGC(1/-1) BsmI R0134 GAATGC(1/-1) BsaMI, BscCI, BsmI, Mva1269I, Nb.BsmI^
Nb.BsmI^ R0706 GAATGC
PdiI GCC/GGC NaeI R0190 GCC/GGC KroI^, MroNI^, NaeI, NgoAIV^, NgoMIV^
NgoMIV^ R0564 G/CCGGC
PdmI GAANN/NNTTC XmnI R0194 GAANN/NNTTC Asp700I , MroXI, XmnI
PenIx GCAGT
PfeI G/AWTC TfiI R0546 G/AWTC TfiI
Pfl1108I x TCGTAG
Pfl23II C/GTACG BsiWI R0553 C/GTACG BsiWI, PspLI , SplI, SunI
PflBI CCANNNN/NTGG PflMI R0509 CCANNNN/NTGG AccB7I , BasI , PflMI, Van91I
PflFI GACN/NNGTC PflFI R0595 GACN/NNGTC AspI , PsyI, TelI, Tth111I
Tth111I R0185 GACN/NNGTC
PflMI CCANNNN/NTGG PflMI R0509 CCANNNN/NTGG AccB7I , BasI , PflBI , Van91I
PfoI T/CCNGGA
PhoI GG/CC PhoI R0705 GG/CC AoxI^, BshFI , BsnI, BspANI, BsuRI, HaeIII, PalI
HaeIII R0108 GG/CC
PinAI A/CCGGT AgeI (1) (2) R0552 A/CCGGT AgeI, AsiAI , BshTI, CspAI
PI-PspI TGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17) PI-PspI R0695 TGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17)
PI-SceI ATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19) PI-SceI R0696 ATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19)
PlaDIx CATCAG(21/19)
Ple19I CGAT/CG PvuI (1) R0150 CGAT/CG BpvUI, BspCI, MvrI, PvuI
PleI GAGTC(4/5) PleI R0515 GAGTC(4/5) MlyI^, Nt.BstNBI^, PpsI , SchI^
MlyI^ R0610 GAGTC(5/5)
Nt.BstNBI^ R0607 GAGTC(4/-5)
PluTI GGCGC/C PluTI R0713 GGCGC/C BbeI , DinI^, EgeI^, KasI^, Mly113I^, NarI^, SfoI^, SspDI^
KasI^ R0544 G/GCGCC
NarI^ R0191 GG/CGCC
SfoI^ R0606 GGC/GCC
PmaCI CAC/GTG PmlI R0532 CAC/GTG AcvI , BbrPI , Eco72I, PmlI, PspCI
PmeI GTTT/AAAC PmeI R0560 GTTT/AAAC MssI
PmlI CAC/GTG PmlI R0532 CAC/GTG AcvI , BbrPI , Eco72I, PmaCI , PspCI
PpiI x (7/12)GAACNNNNNCTC(13/8)
PpsI GAGTC(4/5) MlyI^ R0610 GAGTC(5/5) MlyI^, Nt.BstNBI^, PleI, SchI^
Nt.BstNBI^ R0607 GAGTC(4/-5)
PleI R0515 GAGTC(4/5)
Ppu21I YAC/GTR BsaAI R0531 YAC/GTR BsaAI, BstBAI
PpuMI RG/GWCCY PpuMI R0506 RG/GWCCY Psp5II
PpuXI RG/GWCCY PpuMI R0506 RG/GWCCY PpuMI, Psp5II
PscI A/CATGT PciI R0655 A/CATGT BspLU11I, PciI
PshAI GACNN/NNGTC PshAI R0593 GACNN/NNGTC BoxI, BstPAI
PshBI AT/TAAT AseI R0526 AT/TAAT AseI, AsnI , VspI
PsiI TTA/TAA PsiI R0657 TTA/TAA AanI
Psp124BI GAGCT/C Eco53kI^ R0116 GAG/CTC Ecl136II^, Eco53kI^, EcoICRI^, SacI, SstI
SacI (1) R0156 GAGCT/C
Psp1406I AA/CGTT AclI R0598 AA/CGTT AclI
Psp5II RG/GWCCY PpuMI R0506 RG/GWCCY PpuMI
Psp6I /CCWGG BstNI^ R0168 CC/WGG AjnI ^, BciT130I^, BptI^, BseBI^, BsiLI^, Bst2UI^, BstNI^, BstOI^, EcoRII, MvaI^, PspGI
PspGI R0611 /CCWGG
PspAI C/CCGGG SmaI^ R0141 CCC/GGG Cfr9I, SmaI^, TspMI, XmaCI
TspMI R0709 C/CCGGG
PspCI CAC/GTG PmlI R0532 CAC/GTG AcvI , BbrPI , Eco72I, PmaCI , PmlI
PspEI G/GTNACC BstEII  (1) (2) R0162 G/GTNACC BstEII , BstPI, Eco065I, Eco91I, EcoO65I
PspGI /CCWGG PspGI R0611 /CCWGG AjnI ^, BciT130I^, BptI^, BseBI^, BsiLI^, Bst2UI^, BstNI^, BstOI^, EcoRII, MvaI^, Psp6I
BstNI^ R0168 CC/WGG
PspLI C/GTACG BsiWI R0553 C/GTACG BsiWI, Pfl23II , SplI, SunI
PspN4I GGN/NCC NlaIV R0126 GGN/NCC BmiI, BscBI, NlaIV
PspO3Ix GGWC/C AvaII^ R0153 G/GWCC AflI ^, AvaII^, Bme18I^, Eco47I^, SinI^, Vpak11AI^
PspOMI G/GGCCC PspOMI R0653 G/GGCCC ApaI^, Bsp120I
ApaI^ R0114 GGGCC/C
PspOMIIx CGCCCAR(20/18)
PspPI G/GNCC Sau96I R0165 G/GNCC AspS9I , AsuI , BmgT120I^, BsiZI, BspLI^, Cfr13I, FmuI^, Sau96I, UnbI^
PspPRIx CCYCAG(15/13)
PspXI VC/TCGAGB PspXI R0656 VC/TCGAGB
PsrI (7/12)GAACNNNNNNTAC(12/7)
PssIx RGGNC/CY EcoO109I^ R0503 RG/GNCCY DraII^, EcoO109I^
PstI CTGCA/G PstI (1) R0140 CTGCA/G BspMAI
PstNI CAGNNN/CTG AlwNI R0514 CAGNNN/CTG AlwNI, CaiI
PsuI R/GATCY BstYI R0523 R/GATCY BstX2I, BstYI, MflI, XhoII
PsyI GACN/NNGTC PflFI R0595 GACN/NNGTC AspI , PflFI, TelI, Tth111I
Tth111I R0185 GACN/NNGTC
PteI G/CGCGC BssHII R0199 G/CGCGC BsePI, BssHII, PauI
PvuI CGAT/CG PvuI (1) R0150 CGAT/CG BpvUI, BspCI, MvrI, Ple19I
PvuII CAG/CTG PvuII (1) R0151 CAG/CTG
R9896 GCAGC(8/12) BbvI R0173 GCAGC(8/12) BbvI, BseXI, Bst71I, BstV1I
RcaI T/CATGA BspHI R0517 T/CATGA BspHI, CciI, PagI
RceIx CATCGAC(20/18)
RdeGBIx CCGCAG
RdeGBIIx ACCCAG(20/18)
RdeGBIIIx (9/11)TGRYCA(11/9)
RflFIIIx CGCCAG
RgaI GCGAT/CGC AsiSI R0630 GCGAT/CGC AsiSI, SfaAI, SgfI
RigI GGCCGG/CC FseI R0588 GGCCGG/CC FseI
RlaIx VCW
RleAIx CCCACA(12/9)
RpaB5Ix CGRGGAC(20/18)
RpaBIx CCCGCAG(20/18)
RpaIx GTYGGAG(11/9)
RpaTIx GRTGGAG
RruI TCG/CGA NruI^ R0192 TCG/CGA Bsp68I^, BtuMI^, NruI^
RsaI GT/AC RsaI R0167 GT/AC AfaI , Csp6I^, CviQI^
CviQI^ R0639 G/TAC
RsaNI G/TAC CviQI R0639 G/TAC AfaI ^, Csp6I, CviQI, RsaI^
RsaI^ R0167 GT/AC
RseI CAYNN/NNRTG MslI^ R0571 CAYNN/NNRTG MslI^, SmiMI^
Rsr2I CG/GWCCG
RsrII CG/GWCCG RsrII R0501 CG/GWCCG CpoI, CspI, Rsr2I
SacI GAGCT/C SacI (1) R0156 GAGCT/C Ecl136II^, Eco53kI^, EcoICRI^, Psp124BI , SstI
Eco53kI^ R0116 GAG/CTC
SacII CCGC/GG SacII R0157 CCGC/GG Cfr42I, KspI, Sfr303I, SgrBI, SstII
SalI G/TCGAC SalI (1) (2) R0138 G/TCGAC
SanDIx GG/GWCCC
SapI GCTCTTC(1/4) SapI R0569 GCTCTTC(1/4) BspQI, LguI, Nt.BspQI^, PciSI
BspQI R0712 GCTCTTC(1/4)
Nt.BspQI^ R0644 GCTCTTC(1/-7)
SaqAI T/TAA MseI R0525 T/TAA MseI, Tru1I
SatI GC/NGC Fnu4HI R0178 GC/NGC BisI , BlsI ^, BthCI^, Fnu4HI, Fsp4HI, GluI, ItaI
Sau3AI /GATC Sau3AI R0169 /GATC BfuCI, BscFI, Bsp143I, BssMI, BstENII, BstKTI^, BstMBI, DpnI^, Kzo9I, MalI^, MboI, NdeII^
BfuCI R0636 /GATC
DpnI^ R0176 GA/TC
MboI R0147 /GATC
Sau96I G/GNCC Sau96I R0165 G/GNCC AspS9I , AsuI , BmgT120I^, BsiZI, BspLI^, Cfr13I, FmuI^, PspPI , UnbI^
SauIx CC/TNAGG Bsu36I R0524 CC/TNAGG AocI , AxyI , Bse21I, Bsu36I, CvnI, Eco81I
SbfI CCTGCA/GG SbfI (1) R0642 CCTGCA/GG SdaI, Sse8387I
ScaI AGT/ACT ScaI (1) (2) R0122 AGT/ACT Acc113I , AssI , BmcAI^, ZrmI
SchI GAGTC(5/5) MlyI R0610 GAGTC(5/5) MlyI, Nt.BstNBI^, PleI^, PpsI ^
Nt.BstNBI^ R0607 GAGTC(4/-5)
PleI^ R0515 GAGTC(4/5)
ScrFI CC/NGG ScrFI R0110 CC/NGG Bme1390I, BmrFI, BssKI^, BstSCI^, MspR9I, StyD4I^
BssKI^ R0592 /CCNGG
StyD4I^ R0638 /CCNGG
SdaI CCTGCA/GG SbfI (1) R0642 CCTGCA/GG SbfI, Sse8387I
SdeAIx CAGRAG(21/19)
SdeOSIx (11/-1)GACNNNNRTGA(12/-11)
SduI GDGCH/C Bsp1286I R0120 GDGCH/C BmyI, Bsp1286I, MhlI
SecIx C/CNNGG BsaJI R0536 C/CNNGG BsaJI, BseDI, BssECI
SetI ASST/
SexAI A/CCWGGT SexAI R0605 A/CCWGGT CsiI, MabI
SfaAI GCGAT/CGC AsiSI R0630 GCGAT/CGC AsiSI, RgaI, SgfI
SfaNI GCATC(5/9) SfaNI R0172 GCATC(5/9) BmsI, LweI
SfcI C/TRYAG SfcI R0561 C/TRYAG BfmI , BpcI, BstSFI
SfeIx C/TRYAG SfcI R0561 C/TRYAG BfmI , BpcI, BstSFI, SfcI
SfiI GGCCNNNN/NGGCC SfiI R0123 GGCCNNNN/NGGCC
SfoI GGC/GCC SfoI R0606 GGC/GCC BbeI ^, DinI, EgeI, KasI^, Mly113I^, NarI^, PluTI^, SspDI^
KasI^ R0544 G/GCGCC
NarI^ R0191 GG/CGCC
PluTI^ R0713 GGCGC/C
Sfr274I C/TCGAG PaeR7I R0177 C/TCGAG BssHI, PaeR7I, SlaI, StrI, TliI, XhoI
TliI R0612 C/TCGAG
XhoI (2) R0146 C/TCGAG
Sfr303I CCGC/GG SacII R0157 CCGC/GG Cfr42I, KspI, SacII, SgrBI, SstII
SfuI TT/CGAA BstBI R0519 TT/CGAA AsuII , Bpu14I, BsiCI, Bsp119I, BspT104I, BstBI, Csp45I, NspV
SgeI CNNGNNNNNNNNN/NNNN
SgfI GCGAT/CGC AsiSI R0630 GCGAT/CGC AsiSI, RgaI, SfaAI
SgrAI CR/CCGGYG SgrAI R0603 CR/CCGGYG
SgrBI CCGC/GG SacII R0157 CCGC/GG Cfr42I, KspI, SacII, Sfr303I, SstII
SgrDI CG/TCGACG
SgrTIx CCDS(10/14)
SgsI GG/CGCGCC AscI (2) R0558 GG/CGCGCC AscI, PalAI
SimIx GGGTC(-3/0)
SinI G/GWCC AvaII R0153 G/GWCC AflI , AvaII, Bme18I, Eco47I, PspO3I^, Vpak11AI^
SlaI C/TCGAG PaeR7I R0177 C/TCGAG BssHI, PaeR7I, Sfr274I, StrI, TliI, XhoI
TliI R0612 C/TCGAG
XhoI (2) R0146 C/TCGAG
SmaI CCC/GGG SmaI R0141 CCC/GGG Cfr9I^, PspAI ^, TspMI^, XmaCI^
TspMI^ R0709 C/CCGGG
SmiI ATTT/AAAT SwaI R0604 ATTT/AAAT SwaI
SmiMI CAYNN/NNRTG MslI R0571 CAYNN/NNRTG MslI, RseI^
SmlI C/TYRAG SmlI R0597 C/TYRAG
SmoI C/TYRAG
SmuI CCCGC(4/6) FauI R0651 CCCGC(4/6) FauI
SnaBI TAC/GTA SnaBI R0130 TAC/GTA BstSNI, Eco105I
SnaIx GTATAC BstZ17I^ R0594 GTA/TAC BssNAI^, Bst1107I^, BstZ17I^
Sno506Ix GGCCGAG
SpaHI GCATG/C SphI (1) R0182 GCATG/C BbuI , PaeI , SphI
SpeI A/CTAGT SpeI (1) (2) R0133 A/CTAGT AhlI , BcuI
SphI GCATG/C SphI (1) R0182 GCATG/C BbuI , PaeI , SpaHI
SplIx C/GTACG BsiWI R0553 C/GTACG BsiWI, Pfl23II , PspLI , SunI
SpoDIx GCGGRAG
SrfIx GCCC/GGGC
Sse232Ix CG/CCGGCG
Sse8387I CCTGCA/GG SbfI (1) R0642 CCTGCA/GG SbfI, SdaI
Sse8647Ix AG/GWCCT
Sse9I /AATT MluCI R0538 /AATT MluCI, TasI, Tsp509I, TspEI
Tsp509I R0576 /AATT
SseBI AGG/CCT StuI R0187 AGG/CCT AatI , Eco147I, PceI , StuI
SsiI CCGC(-3/-1) AciI R0551 CCGC(-3/-1) AciI, BspACI
SspBIx T/GTACA BsrGI R0575 T/GTACA AauI , Bsp1407I, BsrGI, BstAUI
SspDI G/GCGCC KasI R0544 G/GCGCC BbeI ^, DinI^, EgeI^, KasI, Mly113I^, NarI^, PluTI^, SfoI^
NarI^ R0191 GG/CGCC
PluTI^ R0713 GGCGC/C
SfoI^ R0606 GGC/GCC
SspI AAT/ATT SspI (1) R0132 AAT/ATT
SstE37Ix CGAAGAC(20/18)
SstI GAGCT/C Eco53kI^ R0116 GAG/CTC Ecl136II^, Eco53kI^, EcoICRI^, Psp124BI , SacI
SacI (1) R0156 GAGCT/C
SstII CCGC/GG SacII R0157 CCGC/GG Cfr42I, KspI, SacII, Sfr303I, SgrBI
Sth132Ix CCCG(4/8)
Sth20745III GGACGAC
StrIx C/TCGAG PaeR7I R0177 C/TCGAG BssHI, PaeR7I, Sfr274I, SlaI, TliI, XhoI
TliI R0612 C/TCGAG
XhoI (2) R0146 C/TCGAG
StuI AGG/CCT StuI R0187 AGG/CCT AatI , Eco147I, PceI , SseBI
StyD4I /CCNGG StyD4I R0638 /CCNGG Bme1390I^, BmrFI^, BssKI, BstSCI, MspR9I^, ScrFI^
BssKI R0592 /CCNGG
ScrFI^ R0110 CC/NGG
StyI C/CWWGG StyI (1) R0500 C/CWWGG BssT1I, Eco130I, EcoT14I, ErhI
SunI C/GTACG BsiWI R0553 C/GTACG BsiWI, Pfl23II , PspLI , SplI
SwaI ATTT/AAAT SwaI R0604 ATTT/AAAT SmiI
TaaI ACN/GT HpyCH4III R0618 ACN/GT Bst4CI, HpyCH4III, Tsp4CI
TaiI ACGT/ HpyCH4IV^ R0619 A/CGT HpyCH4IV^, HpySE526I^, MaeII^, TscI
TaqαI T/CGA TaqαI R0149 T/CGA TthHB8I
TaqI T/CGA TaqαI R0149 T/CGA TaqαI, TthHB8I
TaqII GACCGA(11/9)
TasI /AATT MluCI R0538 /AATT MluCI, Sse9I, Tsp509I, TspEI
Tsp509I R0576 /AATT
TatI W/GTACW
TauI GCSG/C
TelI GACN/NNGTC PflFI R0595 GACN/NNGTC AspI , PflFI, PsyI, Tth111I
Tth111I R0185 GACN/NNGTC
TfiI G/AWTC TfiI R0546 G/AWTC PfeI
ThaI CG/CG BstUI R0518 CG/CG AccII , Bsh1236I, BspFNI^, BstFNI, BstUI, FnuDII^, MvnI
TliI C/TCGAG TliI R0612 C/TCGAG BssHI, PaeR7I, Sfr274I, SlaI, StrI, XhoI
PaeR7I R0177 C/TCGAG
XhoI (2) R0146 C/TCGAG
Tru1I T/TAA MseI R0525 T/TAA MseI, SaqAI
Tru9I T/TAA MseI R0525 T/TAA MseI, SaqAI, Tru1I
TscAI NNCASTGNN/ TspRI R0582 NNCASTGNN/ TspRI
TscI ACGT/ HpyCH4IV^ R0619 A/CGT HpyCH4IV^, HpySE526I^, MaeII^, TaiI
TseFI GTSAC/ Tsp45I^ R0583 /GTSAC NmuCI^, Tsp45I^
TseI G/CWGC TseI R0591 G/CWGC ApeKI
ApeKI R0643 G/CWGC
TsoIx TARCCA(11/9)
Tsp45I /GTSAC Tsp45I R0583 /GTSAC NmuCI, TseFI^
Tsp4CIx ACN/GT HpyCH4III R0618 ACN/GT Bst4CI, HpyCH4III, TaaI
Tsp509I /AATT Tsp509I R0576 /AATT MluCI, Sse9I, TasI, TspEI
MluCI R0538 /AATT
TspDTI ATGAA(11/9)
TspEIx /AATT MluCI R0538 /AATT MluCI, Sse9I, TasI, Tsp509I
Tsp509I R0576 /AATT
TspEIx /AATT MluCI R0538 /AATT MluCI, Sse9I, TasI, Tsp509I, TspEI
Tsp509I R0576 /AATT
TspGWI ACGGA(11/9)
TspMI C/CCGGG TspMI R0709 C/CCGGG Cfr9I, PspAI , SmaI^, XmaCI
SmaI^ R0141 CCC/GGG
TspRI NNCASTGNN/ TspRI R0582 NNCASTGNN/ TscAI
TssIx GAGNNNCTC
TstIx (8/13)CACNNNNNNTCC(12/7)
TsuIx GCGAC
Tth111I GACN/NNGTC Tth111I R0185 GACN/NNGTC AspI , PflFI, PsyI, TelI
PflFI R0595 GACN/NNGTC
Tth111IIx CAARCA(11/9)
TthHB8I T/CGA TaqαI R0149 T/CGA TaqαI
UbaF11Ix TCGTA
UbaF12Ix CTACNNNGTC
UbaF13Ix GAGNNNNNNCTGG
UbaF14Ix CCANNNNNTCG
UbaF9Ix TACNNNNNRTGT
UbaPIx CGAACG
UnbIx /GGNCC Sau96I^ R0165 G/GNCC AspS9I ^, AsuI ^, BmgT120I^, BsiZI^, BspLI^, Cfr13I^, FmuI^, PspPI ^, Sau96I^
Van91I CCANNNN/NTGG PflMI R0509 CCANNNN/NTGG AccB7I , BasI , PflBI , PflMI
Vha464I C/TTAAG AflII R0520 C/TTAAG AflII, BfrI , BspTI, Bst98I, BstAFI, MspCI
VneI G/TGCAC ApaLI R0507 G/TGCAC Alw44I , ApaLI
Vpak11AIx /GGWCC AvaII^ R0153 G/GWCC AflI ^, AvaII^, Bme18I^, Eco47I^, PspO3I^, SinI^
VpaK11BI G/GWCC AvaII R0153 G/GWCC AflI , AvaII, Bme18I, Eco47I, PspO3I^, SinI, Vpak11AI^
VspI AT/TAAT AseI R0526 AT/TAAT AseI, AsnI , PshBI
WviIx CACRAG(20/18)
XagI CCTNN/NNNAGG EcoNI R0521 CCTNN/NNNAGG BstENI, EcoNI
XapI R/AATTY ApoI R0566 R/AATTY AcsI , ApoI
XbaI T/CTAGA XbaI (2) R0145 T/CTAGA
XceI RCATG/Y NspI R0602 RCATG/Y BstNSI, NspI
XcmI CCANNNNN/NNNNTGG XcmI R0533 CCANNNNN/NNNNTGG
XhoI C/TCGAG XhoI (2) R0146 C/TCGAG BssHI, PaeR7I, Sfr274I, SlaI, StrI, TliI
PaeR7I R0177 C/TCGAG
TliI R0612 C/TCGAG
XhoII R/GATCY BstYI R0523 R/GATCY BstX2I, BstYI, MflI, PsuI
XmaCI C/CCGGG SmaI^ R0141 CCC/GGG Cfr9I, PspAI , SmaI^, TspMI
TspMI R0709 C/CCGGG
XmaI C/CCGGG XmaI R0180 C/CCGGG Cfr9I, PspAI , SmaI^, TspMI, XmaCI
TspMI R0709 C/CCGGG
XmaIIIx C/GGCCG EagI (1) R0505 C/GGCCG BseX3I , BstZI, EagI, EclXI, Eco52I
XmaJI C/CTAGG AvrII R0174 C/CTAGG AspA2I , AvrII, BlnI , BspA2I
XmiI GT/MKAC AccI R0161 GT/MKAC AccI, FblI
XmnI GAANN/NNTTC XmnI R0194 GAANN/NNTTC Asp700I , MroXI, PdmI
XspI C/TAG BfaI R0568 C/TAG BfaI, FspBI, MaeI
ZhoI AT/CGAT BspDI R0557 AT/CGAT BanIII , Bsa29I, BseCI, BshVI, BsiXI, Bsp106I, BspDI, BspXI, Bsu15I, BsuTUI, ClaI
ClaI R0197 AT/CGAT
ZraI GAC/GTC ZraI R0659 GAC/GTC AatII^
AatII^ R0117 GACGT/C
ZrmI AGT/ACT ScaI (1) (2) R0122 AGT/ACT Acc113I , AssI , BmcAI^, ScaI
Zsp2I ATGCA/T NsiI R0127 ATGCA/T AvaIII ^, BfrBl, EcoT22I, Mph1103I, NsiI

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