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Invitrogen Platinum SYBR Green qPCR SuperMix-UDG荧光定量试剂 11733046
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  • 货号 11733046
  • 品牌 Invitrogen
  • CAS号
  • 规格/包装 20ul1250次/12.5ml
  • 售卖单位
  • 储存条件 -20℃
  • 现货状态 一个工作日

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Platinum® SYBR® Green qPCR SuperMix-UDG


带有 ROX  的 Platinum® SYBR® Green qPCR SuperMix-UDG  是一种即用型实时定量 PCR (qPCR)  混合试剂,已包含ROX,可在支持  ROX 参考染料的仪器中用于DNA的扩增与检测。产品包括  SYBR Green I  荧光染料和方便即用的  SuperMix  配方,可在很广的目的浓度范围内提供极高灵敏度的目的序列定量检测。混合试剂配方及  UDG  防止残余污染技术也使该试剂适用于高通量定量 PCR。

产品特点

防污染能力强: SuperMix  中的 UDG   dUTP  可阻止前次反应的残余 PCR  产物的重复扩增

特异性强:特异性单克隆抗体重组Taq DNA聚合酶,扩增特异性更强。

检测范围广:可在较宽的模板浓度范围内进行稳定扩增

重复性好:cDNA样品经复孔qPCR扩增检测,显示出良好的重复性


订购信息

产品名称

规格

货号

Platinum® SYBR®  Green qPCR SuperMix-UDG

20uL 1250/12.5mL

11733046

Platinum® SYBR®  Green qPCR SuperMix-UDG with ROX

20uL 1250/12.5mL

11744500


描述

12.5ml,50ul体系500次反应,20ul体系1250次反应
**********************
qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA. ROX reference dye is included in the mix for maximum convenience. These qPCR SuperMixes include:

规格

Detection Method: SYBR
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Product Line: Platinum®, SYBR®
Product Size: 50ul500次/20ul1250次/12.5ml
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Dry Ice

内容及储存

Contains 2X Platinum SYBR Green qPCR SuperMix-UDG with ROX (12.5 mL) and 50 mM Magnesium Chloride (2 × 1 mL).
Contains sufficient reagents for 500 reactions of 50 µL each. Store in freezer (-5 to -30°C).


–Platinum® Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)

–Uracil-DNA Glycosylase (UDG): prevents carryover contamination in conjunction with dUTP (included) for improved specificity
–ROX reference dye premixed at optimal concentration for ABI instruments


qPCR SuperMixes with ROX are available in two formats: 

–Platinum® SYBR® Green qPCR SuperMix-UDG with ROX provides a sensitive, cost-effective system for real-time PCR detection with SYBR® Green I dye (Figure 2)
–Platinum® qPCR SuperMix-UDG with ROX offers superior performance with either fluorogenic primers (e.g., LUX™ Primers) or probe-based detection systems (e.g., TaqMan®) 

Storage:

Store all components at -20°C. Guaranteed stable for 6 months when properly stored.
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