产品名：重组人肿瘤坏死因子TNF-α

产品号：LT0212

产品信息：

TNF-α可下调未成熟DC的巨胞饮作用和表面Fc受体的表达，上调细胞表面MHC Ⅰ类、Ⅱ类分子和B7家族分子（CD80，CD86等）的表达，使未成熟DC分化为成熟DC(mature DC), 此时DC的抗原摄取和加工能力明显减弱，而抗原递呈能力显著增强，可极强的激活T细胞。TNF刺激单核细胞和巨噬分泌IL-1，增强IL-2依赖的胸腺细胞、T细胞增殖能力，促进IL-2、CSF和IFN-γ等淋巴因子产生，增强有丝分裂原或外来抗原刺激B细胞的增殖和Ig分泌。TNF-α在体内、体外均能杀死某些肿瘤细胞，或抑制增殖作用。用防线菌素D、丝裂霉素C、放线菌酮等处理肿瘤细胞（如小鼠成纤维细胞株L929）可明显增TNF-α杀伤肿瘤细胞活性。提高中性粒细胞的吞噬能力，增加过氧化物阴离子产生，增强ADCC功能，刺激细胞脱颗粒和分泌髓过氧化物酶。

别名：

Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

英文描述：

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

表达宿主来源：

E.coli

氨基酸序列：

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

使用建议：

建议使用浓度：20-50ng/ml或500-1000U/ml。

保存条件：

4℃保存6个月，-20℃保存一年或-80℃长期保存。

纯度&内毒素：

Greater than 95% by SDS-PAGE

gel and HPLC analyses.

Endotoxin Level<0.5EU/μg

The purity of rhTNF-α was determined by SDS-PAGE of reduced rhTNF-α and staining overnight with Coomassie Blue.

生物活性：

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.