参考资料：细胞凋亡检测方法指南（PDF）（点击查看）

说明书：Caspase-3/7 Z-DEVD-R110 HTS Assay Kit （点击查看）

Caspase 是一个在细胞凋亡过程中起重要作用的蛋白酶家族。在正常的细胞内，caspase 是以一种无活性状态的前体存在。当细胞发生凋亡时，在凋亡信号的作用下caspase 被激活，引发caspase 酶级联反应，然后通过活化的caspase 裂解特异性底物导致细胞凋亡。
Caspase-3/7 蛋白酶底物是一种罗丹明110 双酰胺衍生物（Z-DEVD-R110），天冬氨酸残基共价地结合在罗丹明110 的氨基酸位点，因此荧光染料罗丹明110可见的吸收光及荧光受到抑制。一旦活化的caspase-3/7 酶切开了底物DEVD肽序列上C-末端的天冬氨酸残基后，在488 nm 激光波长激发下，罗丹明110发出荧光信号。

The Caspase-3/7 Z-DEVD-R110 HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a single-step homogenous assay system for fast and highly sensitive detection of caspase-3/7 activity. The kit can be used to continuously measure the activity of caspase-3/7 in cell extracts and purified enzyme preparations, using a fluorometer or fluorescence microplate reader.
The rhodamine 110-derived substrate (Z-DEVD-R110) used in this assay is a non-fluorescent bisamide compound that, upon enzymatic cleavage, is converted in a two-step process to the fluorescent monoamide and then to the even more fluorescent R110 product. Both of these hydrolysis products exhibit spectral properties similar to those of fluorescein, with peak excitation and emission wavelengths of 496 nm and 520 nm, respectively.
In addition to the Z-DEVD–R110 substrate, the Caspase-3/7 Z-DEVD-R110 Assay Kit contains the reversible aldehyde inhibitor Ac-DEVD-CHO, as well as the reference standard R110. The Ac-DEVD-CHO inhibitor confirms that the fluorescence signals in both induced and control cell populations are due to the activity of caspase-3/7. The reference standard allows for quantification of the amount of R110 released in the reaction.

产品特点:

• HTS-compatible: Single-step homogenous assay specifically designed for HTS-based detection.
• Fast: Fast enzyme kinetics.
• Sensitive: Green fluorescent readout.

货号	产品名称(点击可查看)	激发/发射波长(nm)	规格	价格(¥)
A042	Caspase-3/7 Substrate   Z-DEVD-R110（底物）	496/520	5 mg	4000
A043A A043B	Caspase-3/7 Inhibitor   Ac-DEVD-CHO（抑制剂）	–	1 mg 5 mg	800 2500
A044	Caspase-3/7 Z-DEVD-R110 Assay Kit	496/520	100 assays	2000
A045	Caspase-3/7 Z-DEVD-R110 HTS Assay Kit	496/520	100 assays	2600

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